New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica

F. Alberto; L. Correia; S. Arnaud-Haond; C. Billot; C. M. Duarte; E. Serrão

doi:10.1046/j.1471-8286.2003.00415.x

New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica

F. Alberto^*, L. Correia, S. Arnaud-Haond, C. Billot, C. M. Duarte, E. Serrão

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an important role in coastal ecosystem dynamics. Because of this important role and concerns about the observed regression of some meadows, population genetic studies of this species have been promoted. However, the markers used until now were not polymorphic enough to efficiently assess the level and spatial pattern of genetic variability. Hypervariable molecular markers were obtained by screening a genomic library enriched for microsatellite dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic micro-satellites with an encouraging level of variability at the two geographical scales sampled.

Original language	English (US)
Pages (from-to)	253-255
Number of pages	3
Journal	Molecular Ecology Notes
Volume	3
Issue number	2
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00415.x
State	Published - Jun 2003
Externally published	Yes

Keywords

Clonal plant
Dinucleotides
Microsatellites
Posidoniaceae
Seagrass

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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title = "New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica",

abstract = "The seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an important role in coastal ecosystem dynamics. Because of this important role and concerns about the observed regression of some meadows, population genetic studies of this species have been promoted. However, the markers used until now were not polymorphic enough to efficiently assess the level and spatial pattern of genetic variability. Hypervariable molecular markers were obtained by screening a genomic library enriched for microsatellite dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic micro-satellites with an encouraging level of variability at the two geographical scales sampled.",

keywords = "Clonal plant, Dinucleotides, Microsatellites, Posidoniaceae, Seagrass",

author = "F. Alberto and L. Correia and S. Arnaud-Haond and C. Billot and Duarte, {C. M.} and E. Serr{\~a}o",

year = "2003",

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volume = "3",

pages = "253--255",

journal = "Molecular Ecology Notes",

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T1 - New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica

AU - Alberto, F.

AU - Correia, L.

AU - Arnaud-Haond, S.

AU - Billot, C.

AU - Duarte, C. M.

AU - Serrão, E.

PY - 2003/6

Y1 - 2003/6

N2 - The seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an important role in coastal ecosystem dynamics. Because of this important role and concerns about the observed regression of some meadows, population genetic studies of this species have been promoted. However, the markers used until now were not polymorphic enough to efficiently assess the level and spatial pattern of genetic variability. Hypervariable molecular markers were obtained by screening a genomic library enriched for microsatellite dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic micro-satellites with an encouraging level of variability at the two geographical scales sampled.

AB - The seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an important role in coastal ecosystem dynamics. Because of this important role and concerns about the observed regression of some meadows, population genetic studies of this species have been promoted. However, the markers used until now were not polymorphic enough to efficiently assess the level and spatial pattern of genetic variability. Hypervariable molecular markers were obtained by screening a genomic library enriched for microsatellite dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic micro-satellites with an encouraging level of variability at the two geographical scales sampled.

KW - Clonal plant

KW - Dinucleotides

KW - Microsatellites

KW - Posidoniaceae

KW - Seagrass

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DO - 10.1046/j.1471-8286.2003.00415.x

M3 - Article

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SN - 1471-8278

VL - 3

SP - 253

EP - 255

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica

Abstract

Keywords

ASJC Scopus subject areas

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