TY - CHAP
T1 - NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-infections of Multiple Respiratory Viruses
AU - Bi, Chongwei
AU - Ramos Mandujano, Gerardo
AU - Li, Mo
N1 - KAUST Repository Item: Exported on 2022-09-14
PY - 2022/7/16
Y1 - 2022/7/16
N2 - Detection and mutation surveillance of SARS-CoV-2 are crucial for combating the COVID-19 pandemic. Here we describe a lab-based method for multiplex isothermal amplification-based sequencing and real-time analysis of multiple viral genomes. It can simultaneously detect SARS-CoV-2, influenza A, human adenovirus, and human coronavirus and monitor mutations for up to 96 samples in real time. The method proved to be rapid and sensitive (limit of detection: 29 viral RNA copies/μL of extracted nucleic acid) in detecting SARS-CoV-2 in clinical samples. We expect it to offer a promising solution for rapid field-deployable detection and mutational surveillance of pandemic viruses.
AB - Detection and mutation surveillance of SARS-CoV-2 are crucial for combating the COVID-19 pandemic. Here we describe a lab-based method for multiplex isothermal amplification-based sequencing and real-time analysis of multiple viral genomes. It can simultaneously detect SARS-CoV-2, influenza A, human adenovirus, and human coronavirus and monitor mutations for up to 96 samples in real time. The method proved to be rapid and sensitive (limit of detection: 29 viral RNA copies/μL of extracted nucleic acid) in detecting SARS-CoV-2 in clinical samples. We expect it to offer a promising solution for rapid field-deployable detection and mutational surveillance of pandemic viruses.
UR - http://hdl.handle.net/10754/680021
UR - https://link.springer.com/10.1007/978-1-0716-2395-4_6
UR - http://www.scopus.com/inward/record.url?scp=85134570291&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2395-4_6
DO - 10.1007/978-1-0716-2395-4_6
M3 - Chapter
C2 - 35838953
SN - 9781071623947
SP - 79
EP - 88
BT - Multiplex Biomarker Techniques
PB - Springer US
ER -