Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Chenjie Xu; Keming Xu; Hongwei Gu; Xiaofen Zhong; Zhihong Guo; Rongkun Zheng; Xixiang Zhang; Bing Xu

doi:10.1021/ja031776d

Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Chenjie Xu, Keming Xu, Hongwei Gu, Xiaofen Zhong, Zhihong Guo, Rongkun Zheng, Xixiang Zhang, Bing Xu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

473 Scopus citations

Abstract

Using N_α,N_α-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni²⁺ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

Original language	English (US)
Pages (from-to)	3392-3393
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	11
DOIs	https://doi.org/10.1021/ja031776d
State	Published - Mar 22 2004
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins",

abstract = "Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.",

author = "Chenjie Xu and Keming Xu and Hongwei Gu and Xiaofen Zhong and Zhihong Guo and Rongkun Zheng and Xixiang Zhang and Bing Xu",

year = "2004",

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T1 - Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

AU - Xu, Chenjie

AU - Xu, Keming

AU - Gu, Hongwei

AU - Zhong, Xiaofen

AU - Guo, Zhihong

AU - Zheng, Rongkun

AU - Zhang, Xixiang

AU - Xu, Bing

PY - 2004/3/22

Y1 - 2004/3/22

N2 - Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

AB - Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

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U2 - 10.1021/ja031776d

DO - 10.1021/ja031776d

M3 - Article

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AN - SCOPUS:1642355317

SN - 0002-7863

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 11

ER -

Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Abstract

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