Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos

Angel Raya; Yasuhiko Kawakami; Concepción Rodríguez-Esteban; Dirk Büscher; Christopher M. Koth; Tohru Itoh; Masanobu Morita; R. Marina Raya; Ilir Dubova; Joaquín Grego Bessa; José Luis De la Pompa; Juan Carlos Izpisúa Belmonte

doi:10.1101/gad.1084403

Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos

Angel Raya, Yasuhiko Kawakami, Concepción Rodríguez-Esteban, Dirk Büscher, Christopher M. Koth, Tohru Itoh, Masanobu Morita, R. Marina Raya, Ilir Dubova, Joaquín Grego Bessa, José Luis De la Pompa, Juan Carlos Izpisúa Belmonte^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.

Original language	English (US)
Pages (from-to)	1213-1218
Number of pages	6
Journal	Genes and Development
Volume	17
Issue number	10
DOIs	https://doi.org/10.1101/gad.1084403
State	Published - May 15 2003
Externally published	Yes

Keywords

Left-right asymmetry
Nodal
Notch signaling
Pitx2
Sonic hedgehog
Zebrafish

ASJC Scopus subject areas

General Medicine

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10.1101/gad.1084403

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Raya, A., Kawakami, Y., Rodríguez-Esteban, C., Büscher, D., Koth, C. M., Itoh, T., Morita, M., Raya, R. M., Dubova, I., Bessa, J. G., De la Pompa, J. L., & Izpisúa Belmonte, J. C. (2003). Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos. Genes and Development, 17(10), 1213-1218. https://doi.org/10.1101/gad.1084403

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title = "Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos",

abstract = "Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.",

keywords = "Left-right asymmetry, Nodal, Notch signaling, Pitx2, Sonic hedgehog, Zebrafish",

author = "Angel Raya and Yasuhiko Kawakami and Concepci{\'o}n Rodr{\'i}guez-Esteban and Dirk B{\"u}scher and Koth, {Christopher M.} and Tohru Itoh and Masanobu Morita and Raya, {R. Marina} and Ilir Dubova and Bessa, {Joaqu{\'i}n Grego} and {De la Pompa}, {Jos{\'e} Luis} and {Izpis{\'u}a Belmonte}, {Juan Carlos}",

year = "2003",

month = may,

day = "15",

doi = "10.1101/gad.1084403",

language = "English (US)",

volume = "17",

pages = "1213--1218",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

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Raya, A, Kawakami, Y, Rodríguez-Esteban, C, Büscher, D, Koth, CM, Itoh, T, Morita, M, Raya, RM, Dubova, I, Bessa, JG, De la Pompa, JL & Izpisúa Belmonte, JC 2003, 'Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos', Genes and Development, vol. 17, no. 10, pp. 1213-1218. https://doi.org/10.1101/gad.1084403

TY - JOUR

T1 - Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos

AU - Raya, Angel

AU - Kawakami, Yasuhiko

AU - Rodríguez-Esteban, Concepción

AU - Büscher, Dirk

AU - Koth, Christopher M.

AU - Itoh, Tohru

AU - Morita, Masanobu

AU - Raya, R. Marina

AU - Dubova, Ilir

AU - Bessa, Joaquín Grego

AU - De la Pompa, José Luis

AU - Izpisúa Belmonte, Juan Carlos

PY - 2003/5/15

Y1 - 2003/5/15

N2 - Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.

AB - Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.

KW - Left-right asymmetry

KW - Nodal

KW - Notch signaling

KW - Pitx2

KW - Sonic hedgehog

KW - Zebrafish

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DO - 10.1101/gad.1084403

M3 - Article

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AN - SCOPUS:0037598796

SN - 0890-9369

VL - 17

SP - 1213

EP - 1218

JO - Genes and Development

JF - Genes and Development

IS - 10

ER -

Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos

Abstract

Keywords

ASJC Scopus subject areas

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