Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom‐thrB operon

O. P. Peoples; W. Liebl; M. Bodis; P. J. Maeng; M. T. Follettie; J. A. Archer; A. J. Sinskey

doi:10.1111/j.1365-2958.1988.tb00007.x

Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom‐thrB operon

O. P. Peoples^*, W. Liebl, M. Bodis, P. J. Maeng, M. T. Follettie, J. A. Archer, A. J. Sinskey

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The complete nucleotide sequence of the Corynebacterium glutamicum hom‐thrB operon has been determined and the structural genes and promoter region mapped. A polypeptide of M_r 46136 is encoded by hom and a polypeptide of M_r, 32618 is encoded by thrB. Both predicted protein sequences show amino acid sequence homology to their counterparts in Escherichia coli and Bacillus subtilis. The promoter region has been mapped by S1‐nuclease and deletion analysis. Located between −88, RNA start site and −219 (smallest deletion clone with complete activity) are sequence elements similar to those found in E. coli and B. subtills promoters. Although there are no obvious attenuator‐like structures in the 5′‐untranslated region, there is a dyad‐symmetry element, which may act as an operator.

Original language	English (US)
Pages (from-to)	63-72
Number of pages	10
Journal	Molecular Microbiology
Volume	2
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2958.1988.tb00007.x
State	Published - Jan 1988
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1111/j.1365-2958.1988.tb00007.x

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title = "Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom‐thrB operon",

abstract = "The complete nucleotide sequence of the Corynebacterium glutamicum hom‐thrB operon has been determined and the structural genes and promoter region mapped. A polypeptide of Mr 46136 is encoded by hom and a polypeptide of Mr, 32618 is encoded by thrB. Both predicted protein sequences show amino acid sequence homology to their counterparts in Escherichia coli and Bacillus subtilis. The promoter region has been mapped by S1‐nuclease and deletion analysis. Located between −88, RNA start site and −219 (smallest deletion clone with complete activity) are sequence elements similar to those found in E. coli and B. subtills promoters. Although there are no obvious attenuator‐like structures in the 5′‐untranslated region, there is a dyad‐symmetry element, which may act as an operator.",

author = "Peoples, {O. P.} and W. Liebl and M. Bodis and Maeng, {P. J.} and Follettie, {M. T.} and Archer, {J. A.} and Sinskey, {A. J.}",

year = "1988",

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doi = "10.1111/j.1365-2958.1988.tb00007.x",

language = "English (US)",

volume = "2",

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journal = "Molecular Microbiology",

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T1 - Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom‐thrB operon

AU - Peoples, O. P.

AU - Liebl, W.

AU - Bodis, M.

AU - Maeng, P. J.

AU - Follettie, M. T.

AU - Archer, J. A.

AU - Sinskey, A. J.

PY - 1988/1

Y1 - 1988/1

N2 - The complete nucleotide sequence of the Corynebacterium glutamicum hom‐thrB operon has been determined and the structural genes and promoter region mapped. A polypeptide of Mr 46136 is encoded by hom and a polypeptide of Mr, 32618 is encoded by thrB. Both predicted protein sequences show amino acid sequence homology to their counterparts in Escherichia coli and Bacillus subtilis. The promoter region has been mapped by S1‐nuclease and deletion analysis. Located between −88, RNA start site and −219 (smallest deletion clone with complete activity) are sequence elements similar to those found in E. coli and B. subtills promoters. Although there are no obvious attenuator‐like structures in the 5′‐untranslated region, there is a dyad‐symmetry element, which may act as an operator.

AB - The complete nucleotide sequence of the Corynebacterium glutamicum hom‐thrB operon has been determined and the structural genes and promoter region mapped. A polypeptide of Mr 46136 is encoded by hom and a polypeptide of Mr, 32618 is encoded by thrB. Both predicted protein sequences show amino acid sequence homology to their counterparts in Escherichia coli and Bacillus subtilis. The promoter region has been mapped by S1‐nuclease and deletion analysis. Located between −88, RNA start site and −219 (smallest deletion clone with complete activity) are sequence elements similar to those found in E. coli and B. subtills promoters. Although there are no obvious attenuator‐like structures in the 5′‐untranslated region, there is a dyad‐symmetry element, which may act as an operator.

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U2 - 10.1111/j.1365-2958.1988.tb00007.x

DO - 10.1111/j.1365-2958.1988.tb00007.x

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AN - SCOPUS:0023803688

SN - 0950-382X

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JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 1

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Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom‐thrB operon

Abstract

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