TY - JOUR
T1 - Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
AU - Tehseen, Muhammad
AU - Liao, Chunyan
AU - Dacres, Helen
AU - Dumancic, Mira
AU - Trowell, Stephen
AU - Anderson, Alisha
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PY - 2014/9/25
Y1 - 2014/9/25
N2 - It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
AB - It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
UR - http://hdl.handle.net/10754/334973
UR - http://dx.plos.org/10.1371/journal.pone.0108680
UR - http://www.scopus.com/inward/record.url?scp=84907587228&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0108680
DO - 10.1371/journal.pone.0108680
M3 - Article
C2 - 25254556
SN - 1932-6203
VL - 9
SP - e108680
JO - PLoS ONE
JF - PLoS ONE
IS - 9
ER -