Herein, a robust novel electrochemical sensor for the detection of prophylactic drug Dimetridazole (DMZ) has been developed eco-friendly through the green synthesis of reduced graphene oxide/Prussian blue microcubes (rGO/PB MCs), and the fabrication was economically done by efficient screen-printed carbon electrode (SPCE) modification method. It is critical as DMZ excess level in poultry farm imposes carcinogenic reats.Aresponsive, reproducible and long-lastingDMZsensorwas established using thematerial composed of Prussian blue microcubes encapsulated by thin sheets of reduced graphene oxide (rGO/PB MCs). The rGO/PB MCs composite is prepared through a facile hydrothermal approach, and its elemental, structural, electrochemical and catalyzing abilities are examined. The composite is fabricated on the SPCE, and the resulting improved electrode showed outstanding electrocatalytic ability toward DMZ and the reduction peak current are correlated to the DMZ concentrations. It retains the more extensive working range between 0.02 μM and 1360.1μM and the detection limit reaches 3.2 nM. It also possesses appreciable sensitivity of 2.2935 μAμM-1cm-2. This technique is efficiently applied to the detection of DMZ in spiked samples of milk and egg.