One-pot preparation method for a uniform-sized polymer-based chiral stationary phase for high-performance liquid chromatography with polymethacrylamide as a chiral selector

A simple one-pot method for the preparation of a uniform-sized polymer-based chiral stationary phase (PCSP) for high-performance liquid chromatography was investigated with polymethacrylamide as a chiral selector. During the polymerization process of uniform-sized base particles prepared by a two-step swelling method, solid chiral methacrylamide monomer was added directly to the aqueous polymerization medium of the base particles. The methacrylamide polymerized and was incorporated on the surface of the base polymer particles quantitatively without losing the size uniformity of the base particles. When the methacrylamide was added stepwise, the polymer-based chiral stationary phase obtained gave complete resolution of 2,2′-dihydroxy-1,1′-binaphthyl, whereas lower resolution was observed with PCSPs prepared by the traditional copolymerization method.