TY - JOUR
T1 - Onset of feeding in juvenile sea urchins and its relation to nutrient signalling
AU - Fadl, Alyaa Elsaid Abdelaziz
AU - Mahfouz, Magdy Elsayed
AU - El-Gamal, Mona Mabrouk Taha
AU - Heyland, Andreas
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-25
PY - 2019/1/2
Y1 - 2019/1/2
N2 - The purple sea urchin, Strongylocentrotus purpuratus, has been the focus of extensive ecological and developmental research over the years. Strongylocentrotus purpuratus larvae transition into the juvenile stage after an extensive planktonic period. The metamorphic transition is characterized by dramatic changes in morphology and physiology of the juvenile compared to the larval form and mechanisms underlying this process, especially the early days post-settlement, remain poorly understood. We used SEM and phalloidin stain analysis as well as whole mount in situ hybridization to gain a detailed understanding of juvenile development in relation to nutrient signalling [insulin-like growth factor (IIS), FoxO (forkhead box, sub-group ‘O’) and TOR (target of rampamycin), also known as IIS/TOR/FoxO signalling]. Our results show that the majority of juvenile feeding features are fully developed only after 8-days of juvenile development, leaving an extensive period of nutritional stress. We found that FoxO gene expression increases during that time period and is localized in juvenile tube feet, potentially associated with sensory structures involved in nutrient signalling. Our data complement existing work on sea urchin juvenile development and shed new light on the perimetamorphic period of Strongylocentrotus purpuratus, with respect to nutrient signalling and the potential stressful pre-feeding period of juvenile sea urchins.
AB - The purple sea urchin, Strongylocentrotus purpuratus, has been the focus of extensive ecological and developmental research over the years. Strongylocentrotus purpuratus larvae transition into the juvenile stage after an extensive planktonic period. The metamorphic transition is characterized by dramatic changes in morphology and physiology of the juvenile compared to the larval form and mechanisms underlying this process, especially the early days post-settlement, remain poorly understood. We used SEM and phalloidin stain analysis as well as whole mount in situ hybridization to gain a detailed understanding of juvenile development in relation to nutrient signalling [insulin-like growth factor (IIS), FoxO (forkhead box, sub-group ‘O’) and TOR (target of rampamycin), also known as IIS/TOR/FoxO signalling]. Our results show that the majority of juvenile feeding features are fully developed only after 8-days of juvenile development, leaving an extensive period of nutritional stress. We found that FoxO gene expression increases during that time period and is localized in juvenile tube feet, potentially associated with sensory structures involved in nutrient signalling. Our data complement existing work on sea urchin juvenile development and shed new light on the perimetamorphic period of Strongylocentrotus purpuratus, with respect to nutrient signalling and the potential stressful pre-feeding period of juvenile sea urchins.
UR - https://www.tandfonline.com/doi/full/10.1080/07924259.2018.1513873
UR - http://www.scopus.com/inward/record.url?scp=85053489172&partnerID=8YFLogxK
U2 - 10.1080/07924259.2018.1513873
DO - 10.1080/07924259.2018.1513873
M3 - Article
SN - 0792-4259
VL - 63
SP - 11
EP - 22
JO - Invertebrate Reproduction and Development
JF - Invertebrate Reproduction and Development
IS - 1
ER -