TY - JOUR
T1 - Onsite detection of plant viruses using isothermal amplification assays
AU - Bhat, A I
AU - Aman, Rashid
AU - Mahfouz, Magdy M.
N1 - KAUST Repository Item: Exported on 2022-06-13
Acknowledged KAUST grant number(s): BAS/1/1035-01-01
Acknowledgements: This publication was supported by the King Abdullah University of Science and Technology (KAUST) baseline fund (BAS/1/1035-01-01) to Professor Magdy Mahfouz.
We would like to thank members of genome engineering and synthetic biology laboratory for thoughtful discussions. AIB is thankful to the Science and Engineering Research Board (SERB), Government of India for supporting the project (CRG/2021/000292).
PY - 2022/6/11
Y1 - 2022/6/11
N2 - Plant diseases caused by viruses limit crop production and quality, resulting in significant losses. However, options for managing viruses are limited; for example, as systemic obligate parasites, they cannot be killed by chemicals. Sensitive, robust, affordable diagnostic assays are needed to detect the presence of viruses in plant materials such as seeds, vegetative parts, insect vectors, or alternative hosts, and then prevent or limit their introduction into the field by destroying infected plant materials or controlling insect hosts. Diagnostics based on biological and physical properties are not very sensitive and are time consuming, but assays based on viral proteins and nucleic acids are more specific, sensitive, and rapid. However, most such assays require laboratories with sophisticated equipment and technical skills. By contrast, isothermal-based assays such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are simple, easy to perform, reliable, specific, rapid, and do not require specialized equipment or skills. Isothermal amplification assays can be performed using lateral flow devices, making them suitable for onsite detection or testing in the field. To overcome non-specific amplification and cross-contamination issues, isothermal amplification assays can be coupled with CRISPR/Cas technology. Indeed, the collateral activity associated with some CRISPR/Cas systems has been successfully harnessed for visual detection of plant viruses. Here, we briefly describe traditional methods for detecting viruses, then examine the various isothermal assays that are being harnessed to detect viruses.
AB - Plant diseases caused by viruses limit crop production and quality, resulting in significant losses. However, options for managing viruses are limited; for example, as systemic obligate parasites, they cannot be killed by chemicals. Sensitive, robust, affordable diagnostic assays are needed to detect the presence of viruses in plant materials such as seeds, vegetative parts, insect vectors, or alternative hosts, and then prevent or limit their introduction into the field by destroying infected plant materials or controlling insect hosts. Diagnostics based on biological and physical properties are not very sensitive and are time consuming, but assays based on viral proteins and nucleic acids are more specific, sensitive, and rapid. However, most such assays require laboratories with sophisticated equipment and technical skills. By contrast, isothermal-based assays such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are simple, easy to perform, reliable, specific, rapid, and do not require specialized equipment or skills. Isothermal amplification assays can be performed using lateral flow devices, making them suitable for onsite detection or testing in the field. To overcome non-specific amplification and cross-contamination issues, isothermal amplification assays can be coupled with CRISPR/Cas technology. Indeed, the collateral activity associated with some CRISPR/Cas systems has been successfully harnessed for visual detection of plant viruses. Here, we briefly describe traditional methods for detecting viruses, then examine the various isothermal assays that are being harnessed to detect viruses.
UR - http://hdl.handle.net/10754/678889
UR - https://onlinelibrary.wiley.com/doi/10.1111/pbi.13871
U2 - 10.1111/pbi.13871
DO - 10.1111/pbi.13871
M3 - Article
C2 - 35689490
SN - 1467-7644
JO - Plant biotechnology journal
JF - Plant biotechnology journal
ER -