Optical tweezers for sample fixing in micro-diffraction experiments

H. Amenitsch; D. Cojoc; M. Rappolt; B. Sartori; P. Laggner; E. Ferrari; V. Garbin; M. Burghammer; Ch Riekel; E. Di Fabrizio

doi:10.1063/1.2436299

Optical tweezers for sample fixing in micro-diffraction experiments

H. Amenitsch^*, D. Cojoc, M. Rappolt, B. Sartori, P. Laggner, E. Ferrari, V. Garbin, M. Burghammer, Ch Riekel, E. Di Fabrizio

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

Original language	English (US)
Title of host publication	SYNCHROTRON RADIATION INSTRUMENTATION
Subtitle of host publication	Ninth International Conference on Synchrotron Radiation Instrumentation
Pages	1287-1290
Number of pages	4
DOIs	https://doi.org/10.1063/1.2436299
State	Published - 2007
Externally published	Yes
Event	SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation - Daegu, Korea, Republic of Duration: May 28 2006 → Jun 28 2006

Publication series

Name	AIP Conference Proceedings
Volume	879
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Other

Other	SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation
Country/Territory	Korea, Republic of
City	Daegu
Period	05/28/06 → 06/28/06

Keywords

Optical manipulation
Phospholipids
Single biological entity
X-ray micro diffraction

ASJC Scopus subject areas

General Physics and Astronomy

Access to Document

10.1063/1.2436299

Cite this

Amenitsch, H., Cojoc, D., Rappolt, M., Sartori, B., Laggner, P., Ferrari, E., Garbin, V., Burghammer, M., Riekel, C., & Di Fabrizio, E. (2007). Optical tweezers for sample fixing in micro-diffraction experiments. In SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation (pp. 1287-1290). (AIP Conference Proceedings; Vol. 879). https://doi.org/10.1063/1.2436299

@inproceedings{395c5ad1012840a68e8e353ad6bd550e,

title = "Optical tweezers for sample fixing in micro-diffraction experiments",

abstract = "In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.",

keywords = "Optical manipulation, Phospholipids, Single biological entity, X-ray micro diffraction",

author = "H. Amenitsch and D. Cojoc and M. Rappolt and B. Sartori and P. Laggner and E. Ferrari and V. Garbin and M. Burghammer and Ch Riekel and {Di Fabrizio}, E.",

year = "2007",

doi = "10.1063/1.2436299",

language = "English (US)",

isbn = "0735403732",

series = "AIP Conference Proceedings",

pages = "1287--1290",

booktitle = "SYNCHROTRON RADIATION INSTRUMENTATION",

note = "SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation ; Conference date: 28-05-2006 Through 28-06-2006",

}

Amenitsch, H, Cojoc, D, Rappolt, M, Sartori, B, Laggner, P, Ferrari, E, Garbin, V, Burghammer, M, Riekel, C & Di Fabrizio, E 2007, Optical tweezers for sample fixing in micro-diffraction experiments. in SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation. AIP Conference Proceedings, vol. 879, pp. 1287-1290, SYNCHROTRON RADIATION INSTRUMENTATION: 9th International Conference on Synchrotron Radiation Instrumentation, Daegu, Korea, Republic of, 05/28/06. https://doi.org/10.1063/1.2436299

Optical tweezers for sample fixing in micro-diffraction experiments. / Amenitsch, H.; Cojoc, D.; Rappolt, M. et al.
SYNCHROTRON RADIATION INSTRUMENTATION: Ninth International Conference on Synchrotron Radiation Instrumentation. 2007. p. 1287-1290 (AIP Conference Proceedings; Vol. 879).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Optical tweezers for sample fixing in micro-diffraction experiments

AU - Amenitsch, H.

AU - Cojoc, D.

AU - Rappolt, M.

AU - Sartori, B.

AU - Laggner, P.

AU - Ferrari, E.

AU - Garbin, V.

AU - Burghammer, M.

AU - Riekel, Ch

AU - Di Fabrizio, E.

PY - 2007

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AB - In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 μm large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

KW - Optical manipulation

KW - Phospholipids

KW - Single biological entity

KW - X-ray micro diffraction

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T3 - AIP Conference Proceedings

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Y2 - 28 May 2006 through 28 June 2006

ER -

Optical tweezers for sample fixing in micro-diffraction experiments

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