Osmosedimentation: Approach to sedimentation equilibrium under gravity

Alfredo T.N. Pires*, Suzana P. Nunes, Fernando Galembeck

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Aqueous dextran solutions contained within tall (20 cm) dialysis cells reach a state of sedimentation equilibrium in less than 10 hr, under gravity. Experiments were performed in which the solute was transported downward or upward, giving always the same final result. Calculation of molecular weights (M̄w) of two well-defined dextran samples and of bovine thyroglobulin, using the vertical concentration gradients measured in the equilibrated solution columns, gives results in agreement with other methods. The well-known kinetic limitations to solute transport under gravity are thus effectively removed by coupling solvent osmotic currents to solute sedimentation.

Original languageEnglish (US)
Pages (from-to)489-493
Number of pages5
JournalJournal of colloid and interface science
Volume98
Issue number2
DOIs
StatePublished - Apr 1984
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Osmosedimentation: Approach to sedimentation equilibrium under gravity'. Together they form a unique fingerprint.

Cite this