Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures

Yihua Loo; Anupama Lakshmanan; Ming Ni; Lai Ling Toh; Shi Wang; Charlotte A.E. Hauser

doi:10.1021/acs.nanolett.5b02859

Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures

Yihua Loo, Anupama Lakshmanan, Ming Ni, Lai Ling Toh, Shi Wang, Charlotte A.E. Hauser^*

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

167 Scopus citations

Abstract

Printable scaffolds with adequate mechanical strength and stiffness are sought after to ensure viability of printed cells and tissues. We report the first peptide bioinks-lysine-containing hexapeptides that self-assemble into stable, nanofibrous three-dimensional hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible scaffolds support the three-dimensional culture of human stem cells and differentiation of primary cells into organotypic (gastrointestinal and skin) structures for high-throughput screening, diagnosis, and tissue engineering.

Original language	English (US)
Pages (from-to)	6919-6925
Number of pages	7
Journal	Nano Letters
Volume	15
Issue number	10
DOIs	https://doi.org/10.1021/acs.nanolett.5b02859
State	Published - Oct 14 2015

Keywords

Peptide bioink
bioprinting
nanofibrous scaffold
smart biomaterials
stimuli-responsive self-assembly
ultrashort peptides

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.5b02859

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title = "Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures",

abstract = "Printable scaffolds with adequate mechanical strength and stiffness are sought after to ensure viability of printed cells and tissues. We report the first peptide bioinks-lysine-containing hexapeptides that self-assemble into stable, nanofibrous three-dimensional hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible scaffolds support the three-dimensional culture of human stem cells and differentiation of primary cells into organotypic (gastrointestinal and skin) structures for high-throughput screening, diagnosis, and tissue engineering.",

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AU - Lakshmanan, Anupama

AU - Ni, Ming

AU - Toh, Lai Ling

AU - Wang, Shi

AU - Hauser, Charlotte A.E.

PY - 2015/10/14

Y1 - 2015/10/14

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AB - Printable scaffolds with adequate mechanical strength and stiffness are sought after to ensure viability of printed cells and tissues. We report the first peptide bioinks-lysine-containing hexapeptides that self-assemble into stable, nanofibrous three-dimensional hydrogels with unprecedented stiffness of up to 40 kPa. These biocompatible scaffolds support the three-dimensional culture of human stem cells and differentiation of primary cells into organotypic (gastrointestinal and skin) structures for high-throughput screening, diagnosis, and tissue engineering.

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KW - bioprinting

KW - nanofibrous scaffold

KW - smart biomaterials

KW - stimuli-responsive self-assembly

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Peptide Bioink: Self-Assembling Nanofibrous Scaffolds for Three-Dimensional Organotypic Cultures

Abstract

Keywords

ASJC Scopus subject areas

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