TY - JOUR
T1 - Performance characteristics of a recombinant enzymatic cycling assay for quantification of total homocysteine in serum or plasma
AU - Roberts, Richard F.
AU - Roberts, William L.
N1 - Generated from Scopus record by KAUST IRTS on 2023-09-20
PY - 2004/6/1
Y1 - 2004/6/1
N2 - Background: Homocysteine is an amino acid that has been linked to an increased risk of coronary artery disease and stroke. A recombinant enzymatic cycling assay for homocysteine was evaluated using a Roche Modular Analytics P800 chemistry analyzer. Methods: Bound homocysteine is released by disulfide reduction and combined with serine to form L-cystathionine that is degraded by cystathionine β-lyase into homocysteine, pyruvate, and ammonia. Pyruvate is converted to lactate and the amount of NAD+ produced is directly proportional to the concentration of homocysteine. The limit of detection (LOD), linearity, imprecision, method comparison, reference interval and susceptibility to common interferences were assessed. Results: The limit of detection was 0.31 μmol/l. The method was linear from 1 to 100 μmol/l with a maximum deviation from the target concentration of
AB - Background: Homocysteine is an amino acid that has been linked to an increased risk of coronary artery disease and stroke. A recombinant enzymatic cycling assay for homocysteine was evaluated using a Roche Modular Analytics P800 chemistry analyzer. Methods: Bound homocysteine is released by disulfide reduction and combined with serine to form L-cystathionine that is degraded by cystathionine β-lyase into homocysteine, pyruvate, and ammonia. Pyruvate is converted to lactate and the amount of NAD+ produced is directly proportional to the concentration of homocysteine. The limit of detection (LOD), linearity, imprecision, method comparison, reference interval and susceptibility to common interferences were assessed. Results: The limit of detection was 0.31 μmol/l. The method was linear from 1 to 100 μmol/l with a maximum deviation from the target concentration of
UR - https://linkinghub.elsevier.com/retrieve/pii/S0009898104000981
UR - http://www.scopus.com/inward/record.url?scp=2442472190&partnerID=8YFLogxK
U2 - 10.1016/j.cccn.2004.02.013
DO - 10.1016/j.cccn.2004.02.013
M3 - Article
SN - 0009-8981
VL - 344
SP - 95
EP - 99
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -