Performance of extraction methods for extracellular DNA from sediments across marine habitats

Nathan Geraldi; Rubén Díaz-Rúa; Lauren A. Shea; Carlos M. Duarte

doi:10.1002/edn3.48

Performance of extraction methods for extracellular DNA from sediments across marine habitats

Nathan Geraldi, Rubén Díaz-Rúa, Lauren A. Shea, Carlos M. Duarte

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.

Original language	English (US)
Pages (from-to)	91-98
Number of pages	8
Journal	Environmental DNA
Volume	2
Issue number	1
DOIs	https://doi.org/10.1002/edn3.48
State	Published - Nov 2 2019

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10.1002/edn3.48

https://repository.kaust.edu.sa/bitstream/10754/661405/1/edn3.48.pdf

Cite this

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title = "Performance of extraction methods for extracellular DNA from sediments across marine habitats",

abstract = "Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.",

author = "Nathan Geraldi and Rub{\'e}n D{\'i}az-R{\'u}a and Shea, {Lauren A.} and Duarte, {Carlos M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This research was supported by the King Abdullah University of Science and Technology through baseline funding and funds by the Tarek Ahmed Juffali Research Chair in Red Sea Ecology. We thank A. Anton, P. Carrillo de Albornoz, and J. Martinez Ayala for help-ing to collect samples. All authors declare they have not conflict of interest.",

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doi = "10.1002/edn3.48",

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T1 - Performance of extraction methods for extracellular DNA from sediments across marine habitats

AU - Geraldi, Nathan

AU - Díaz-Rúa, Rubén

AU - Shea, Lauren A.

AU - Duarte, Carlos M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This research was supported by the King Abdullah University of Science and Technology through baseline funding and funds by the Tarek Ahmed Juffali Research Chair in Red Sea Ecology. We thank A. Anton, P. Carrillo de Albornoz, and J. Martinez Ayala for help-ing to collect samples. All authors declare they have not conflict of interest.

PY - 2019/11/2

Y1 - 2019/11/2

N2 - Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.

AB - Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.

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DO - 10.1002/edn3.48

M3 - Article

SN - 2637-4943

VL - 2

SP - 91

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JO - Environmental DNA

JF - Environmental DNA

IS - 1

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Performance of extraction methods for extracellular DNA from sediments across marine habitats

Abstract

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