Pitx2 regulates gonad morphogenesis

Joaquín Rodríguez-León, Concepción Rodríguez Esteban, Mercè Martí, Belén Santiago-Josefat, Ilir Dubova, Xavier Rubiralta, Juan Carlos Izpisúa Belmonte

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Organ shape and size, and, ultimately, organ function, relate in part to the cell and tissue spatial arrangement that takes place during embryonic development. Despite great advances in the genetic regulatory networks responsible for tissue and organ development, it is not yet clearly understood how specific gene functions are linked to the specific morphogenetic processes underlying the internal organ asymmetries found in vertebrate animals. During female chick embryogenesis, and in contrast to males where both testes develop symmetrically, asymmetrical gonad morphogenesis results in only one functional ovary. The disposition of paired organs along the left-right body axis has been shown to be regulated by the activity of the homeobox containing gene pitx2. We have found that pitx2 regulates cell adhesion, affinity, and cell recognition events in the developing gonad primordium epithelia. This in turn not only allows for proper somatic development of the gonad cortex but also permits the proliferation and differentiation of primordial germ cells. We illustrate how Pitx2 activity directs asymmetrical gonad morphogenesis by controlling mitotic spindle orientation of the developing gonad cortex and how, by modulating cyclinD1 expression during asymmetric ovarian development, Pitx2 appears to control gonad organ size. All together our observations indicate that the effects elicited by Pitx2 during the development of the female chick ovary are critical for cell topology, growth, fate, and ultimately organ morphogenesis and function.

Original languageEnglish (US)
Pages (from-to)11242-11247
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number32
StatePublished - Aug 12 2008
Externally publishedYes


  • Chick ovary development
  • Cyclin D1
  • Left-right asymmetry
  • Mitotic orientation
  • Primordial germ cell

ASJC Scopus subject areas

  • General


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