TY - JOUR
T1 - Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway
AU - Weits, Daan A.
AU - Giuntoli, Beatrice
AU - Kosmacz, Monika
AU - Parlanti, Sandro
AU - Hubberten, Hans Michael
AU - Riegler, Heike
AU - Hoefgen, Rainer
AU - Perata, Pierdomenico
AU - Van Dongen, Joost T.
AU - Licausi, Francesco
N1 - Generated from Scopus record by KAUST IRTS on 2021-02-18
PY - 2014/3/6
Y1 - 2014/3/6
N2 - In plant and animal cells, amino-terminal cysteine oxidation controls selective proteolysis via an oxygen-dependent branch of the N-end rule pathway. It remains unknown how the N-terminal cysteine is specifically oxidized. Here we identify plant cysteine oxidase (PCO) enzymes that oxidize the penultimate cysteine of ERF-VII transcription factors by using oxygen as a co-substrate, thereby controlling the lifetime of these proteins. Consequently, ERF-VII proteins are stabilized under hypoxia and activate the molecular response to low oxygen while the expression of anaerobic genes is repressed in air. Members of the PCO family are themselves targets of ERF-VII transcription factors, generating a feedback loop that adapts the stress response according to the extent of the hypoxic condition. Our results reveal that PCOs act as sensor proteins for oxygen in plants and provide an example of how proactive regulation of the N-end rule pathway balances stress response to optimal growth and development in plants. © 2014 Macmillan Publishers Limited. All rights reserved.
AB - In plant and animal cells, amino-terminal cysteine oxidation controls selective proteolysis via an oxygen-dependent branch of the N-end rule pathway. It remains unknown how the N-terminal cysteine is specifically oxidized. Here we identify plant cysteine oxidase (PCO) enzymes that oxidize the penultimate cysteine of ERF-VII transcription factors by using oxygen as a co-substrate, thereby controlling the lifetime of these proteins. Consequently, ERF-VII proteins are stabilized under hypoxia and activate the molecular response to low oxygen while the expression of anaerobic genes is repressed in air. Members of the PCO family are themselves targets of ERF-VII transcription factors, generating a feedback loop that adapts the stress response according to the extent of the hypoxic condition. Our results reveal that PCOs act as sensor proteins for oxygen in plants and provide an example of how proactive regulation of the N-end rule pathway balances stress response to optimal growth and development in plants. © 2014 Macmillan Publishers Limited. All rights reserved.
UR - http://www.nature.com/articles/ncomms4425
UR - http://www.scopus.com/inward/record.url?scp=84896795351&partnerID=8YFLogxK
U2 - 10.1038/ncomms4425
DO - 10.1038/ncomms4425
M3 - Article
SN - 2041-1723
VL - 5
JO - Nature Communications
JF - Nature Communications
ER -