TY - JOUR
T1 - PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance
AU - Burkart, Rebecca C
AU - Strotmann, Vivien I
AU - Kirschner, Gwendolyn Kristin
AU - Akinci, Abdullah
AU - Czempik, Laura
AU - Dolata, Anika
AU - Maizel, Alexis
AU - Weidtkamp-Peters, Stefanie
AU - Stahl, Yvonne
N1 - KAUST Repository Item: Exported on 2022-04-15
Acknowledgements: We would like to acknowledge funding of R.C.B. by the Deutsche Forschungsgemeinschaft (DFG) through grant Sta1212/1-1 to Y.S. We thank Andrea Bleckmann for sharing the Greengate Cerulean construct, Renze Heidstra, and Ben Scheres for sharing published lines (PLT
PY - 2022/4/4
Y1 - 2022/4/4
N2 - Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.
AB - Maintenance and homeostasis of the stem cell niche (SCN) in the Arabidopsis root is essential for growth and development of all root cell types. The SCN is organized around a quiescent center (QC) maintaining the stemness of cells in direct contact. The key transcription factors (TFs) WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and PLETHORAs (PLTs) are expressed in the SCN where they maintain the QC and regulate distal columella stem cell (CSC) fate. Here, we describe the concerted mutual regulation of the key TFs WOX5 and PLTs on a transcriptional and protein interaction level. Additionally, by applying a novel SCN staining method, we demonstrate that both WOX5 and PLTs regulate root SCN homeostasis as they control QC quiescence and CSC fate interdependently. Moreover, we uncover that some PLTs, especially PLT3, contain intrinsically disordered prion-like domains (PrDs) that are necessary for complex formation with WOX5 and its recruitment to subnuclear microdomains/nuclear bodies (NBs) in the CSCs. We propose that this partitioning of PLT-WOX5 complexes to NBs, possibly by phase separation, is important for CSC fate determination.
UR - http://hdl.handle.net/10754/676255
UR - https://onlinelibrary.wiley.com/doi/10.15252/embr.202154105
UR - http://www.scopus.com/inward/record.url?scp=85127407199&partnerID=8YFLogxK
U2 - 10.15252/embr.202154105
DO - 10.15252/embr.202154105
M3 - Article
C2 - 35373503
SN - 1469-3178
JO - EMBO Reports
JF - EMBO Reports
ER -