Point inoculation method for measuring adult plant response of wheat to stripe rust infection

Willem H.P. Boshoff*, Renée Prins, Corneli De Klerk, Simon G. Krattinger, Cornelia M. Bender, Gerrie J. Maree, Lisa Rothmann, Zacharias A. Pretorius

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Depending on the pathogenicity of the stripe rust fungus Puccinia striiformis f. sp. tritici, the nature of resistance in the wheat host plant, and the environment, a broad range of disease phenotypes can be expressed. Therefore, the phenotyping of partial adult plant stripe rust resistance requires reliable and repeatable procedures, especially under controlled conditions. In this study, the development of a flag leaf point inoculation method, which resulted in a 100% initial infection rate, is reported. Flag leaf inoculations were achieved by placing 6-mm antibiotic test paper discs, dipped into a urediniospore and water suspension and covered with water-proof plastic tape, on the adaxial side of leaves. Results from independent trials allowed for the statistical comparison of stripe rust lesion expansion rate in wheat entries that differ in resistance. The technique is inexpensive, reliable, and applicable to routine screening for adult plant response type, quantitative comparison of stripe rust progress, environmental influences, and pathogenicity of different isolates.

Original languageEnglish (US)
Pages (from-to)1228-1233
Number of pages6
JournalPlant Disease
Issue number6
StatePublished - Jun 2019

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


Dive into the research topics of 'Point inoculation method for measuring adult plant response of wheat to stripe rust infection'. Together they form a unique fingerprint.

Cite this