Abstract
A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. Experimental evaluation of this amplitude point spread function has been carried out by using a holographic set-up and 60 nanometers gold spheres as punctual objects. The measured values are then compared with the theoretical predicted model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 202-209 |
| Number of pages | 8 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 5143 |
| DOIs | |
| State | Published - 2003 |
| Externally published | Yes |
| Event | Novel Optical Instrumentation for Biomedical Applications - Munich, Germany Duration: Jun 24 2003 → Jun 25 2003 |
Keywords
- Diffraction
- Digital holographic microscopy
- Optical aberrations
- Point spread function
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering