Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

Cátia Ornelas-Megiatto; Peter R. Wich; Jean Frechet

doi:10.1021/ja207366k

Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

Cátia Ornelas-Megiatto, Peter R. Wich, Jean Frechet

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123 Scopus citations

Abstract

A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.

Original language	English (US)
Pages (from-to)	1902-1905
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	4
DOIs	https://doi.org/10.1021/ja207366k
State	Published - Jan 19 2012

ASJC Scopus subject areas

Biochemistry
Colloid and Surface Chemistry
General Chemistry
Catalysis

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10.1021/ja207366k

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@article{c93b8e35d1b448fca12682c8010392bb,

title = "Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers",

abstract = "A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. {\textcopyright} 2012 American Chemical Society.",

author = "C{\'a}tia Ornelas-Megiatto and Wich, {Peter R.} and Jean Frechet",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This project has been funded in part with Federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN268201000043C, and in part through the Frechet {"}various donors{"} fund for the support of research in new materials. We thank Ann Fischer and Michelle Yasukawa for help with cell culture and Dr. Peter Friebe and Dr. Eva Harris for assistance with and the use of their luminescence plate reader. P.R.W. gratefully acknowledges the Alexander von Humboldt Foundation (AvH) for partial funding.",

year = "2012",

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doi = "10.1021/ja207366k",

language = "English (US)",

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T1 - Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

AU - Ornelas-Megiatto, Cátia

AU - Wich, Peter R.

AU - Frechet, Jean

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This project has been funded in part with Federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN268201000043C, and in part through the Frechet "various donors" fund for the support of research in new materials. We thank Ann Fischer and Michelle Yasukawa for help with cell culture and Dr. Peter Friebe and Dr. Eva Harris for assistance with and the use of their luminescence plate reader. P.R.W. gratefully acknowledges the Alexander von Humboldt Foundation (AvH) for partial funding.

PY - 2012/1/19

Y1 - 2012/1/19

N2 - A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.

AB - A water-soluble polyphosphonium polymer was synthesized and directly compared with its ammonium analog in terms of siRNA delivery. The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viability, whereas the best result obtained for the ammonium analog reaches only 25% transfection with 85% cell viability. Moreover, the nature of the alkyl substituents on the phosphonium cations is shown to have an important influence on the transfection efficiency and toxicity of the polyplexes. The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems. © 2012 American Chemical Society.

UR - http://hdl.handle.net/10754/562078

UR - https://pubs.acs.org/doi/10.1021/ja207366k

UR - http://www.scopus.com/inward/record.url?scp=84856480640&partnerID=8YFLogxK

U2 - 10.1021/ja207366k

DO - 10.1021/ja207366k

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SN - 0002-7863

VL - 134

SP - 1902

EP - 1905

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 4

ER -

Polyphosphonium polymers for siRNA delivery: An efficient and nontoxic alternative to polyammonium carriers

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