Pre-mRNA splicing repression triggers abiotic stress signaling in plants

Yu Ling, Sahar Alshareef, Haroon Butt, Jorge Lozano-Juste, Lixin Li, Aya A. Galal, Ahmed Moustafa, Afaque Ahmad Imtiyaz Momin, Manal Tashkandi, Dale N. Richardson, Hiroaki Fujii, Stefan T. Arold, Pedro L. Rodriguez, Paula Duque, Magdy M. Mahfouz

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A
Original languageEnglish (US)
Pages (from-to)291-309
Number of pages19
JournalThe Plant Journal
Volume89
Issue number2
DOIs
StatePublished - Jan 17 2017

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