TY - JOUR
T1 - Proteome analysis of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk
AU - Janjanam, Jagadeesh
AU - Jamwal, Manu
AU - Singh, Surender V.
AU - Kumar, Saravanan
AU - Panigrahi, Aswini Kumar
AU - Hariprasad, Gururao
AU - Jena, Manoj Kumar
AU - Anand, Vijay R.
AU - Kumar, Sudarshan Suresh
AU - Kaushik, Jai Kumar
AU - Dang, Ajaykumar
AU - Mukesh, Manishi
AU - Mishra, Bishnu Prasad
AU - Srinivasan, Alagiri
AU - Reddy, Vanga Siva Belum
AU - Mohanty, Ashok Kumar
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The research was supported by National Agriculture Innovation Project (component 4), Indian Council of Agricultural Research (ICAR), Government of India. A part of the MS data was collected at Seattle Biomed, Seattle, USA. J.J. received a junior research fellowship from the council of scientific research (CSIR), Government of India. We acknowledge the use of computational facility BTIS, sub center, NDRI.
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
UR - http://hdl.handle.net/10754/563032
UR - http://doi.wiley.com/10.1002/pmic.201300031
UR - http://www.scopus.com/inward/record.url?scp=84887104005&partnerID=8YFLogxK
U2 - 10.1002/pmic.201300031
DO - 10.1002/pmic.201300031
M3 - Article
C2 - 24030930
SN - 1615-9853
VL - 13
SP - 3189
EP - 3204
JO - PROTEOMICS
JF - PROTEOMICS
IS - 21
ER -