Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno

Nicola Storelli; Maged M. Saad; Niels Ulrik Frigaard; Xavier Perret; Mauro Tonolla

doi:10.1016/j.euprot.2013.11.010

Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16^T isolated from Lake Cadagno

Nicola Storelli^*, Maged M. Saad, Niels Ulrik Frigaard, Xavier Perret, Mauro Tonolla

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16^T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO₂ assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

Original language	English (US)
Pages (from-to)	17-30
Number of pages	14
Journal	EuPA Open Proteomics
Volume	2
DOIs	https://doi.org/10.1016/j.euprot.2013.11.010
State	Published - Mar 2014
Externally published	Yes

Keywords

Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle
Granules of poly(3-hydroxybutyrate) (PHB)
Phototrophic sulfur bacteria

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1016/j.euprot.2013.11.010

Cite this

@article{df110d148c1e434db180a38f5809877d,

title = "Proteomic analysis of the purple sulfur bacterium Candidatus {"}Thiodictyon syntrophicum{"} strain Cad16T isolated from Lake Cadagno",

abstract = "Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus {"}Thiodictyon syntrophicum{"} strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.",

keywords = "Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle, Granules of poly(3-hydroxybutyrate) (PHB), Phototrophic sulfur bacteria",

author = "Nicola Storelli and Saad, {Maged M.} and Frigaard, {Niels Ulrik} and Xavier Perret and Mauro Tonolla",

note = "Funding Information: Financial support for this project was provided by the University of Geneva, the Institute of Microbiology and State of Ticino, and the Swiss National Science Foundation (grant no. 31003A-116591 ). The authors are indebted to Cinzia Benagli, Sophie De Respinis and Natalia Giot for technical and moral support. Furthermore, we are grateful to D. Burri and M. Wittwer from SPIEZ Laboratory, Federal Office for Civil Protection (FOCP), Spiez (Switzlerland), for their technical support. The authors would also like to thank the FEMS and Alpine Biology Center Foundation (ABC) for their support.",

year = "2014",

month = mar,

doi = "10.1016/j.euprot.2013.11.010",

language = "English (US)",

volume = "2",

pages = "17--30",

journal = "EuPA Open Proteomics",

issn = "2212-9685",

publisher = "Elsevier B.V.",

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T1 - Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno

AU - Storelli, Nicola

AU - Saad, Maged M.

AU - Frigaard, Niels Ulrik

AU - Perret, Xavier

AU - Tonolla, Mauro

N1 - Funding Information: Financial support for this project was provided by the University of Geneva, the Institute of Microbiology and State of Ticino, and the Swiss National Science Foundation (grant no. 31003A-116591 ). The authors are indebted to Cinzia Benagli, Sophie De Respinis and Natalia Giot for technical and moral support. Furthermore, we are grateful to D. Burri and M. Wittwer from SPIEZ Laboratory, Federal Office for Civil Protection (FOCP), Spiez (Switzlerland), for their technical support. The authors would also like to thank the FEMS and Alpine Biology Center Foundation (ABC) for their support.

PY - 2014/3

Y1 - 2014/3

N2 - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

AB - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

KW - Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle

KW - Granules of poly(3-hydroxybutyrate) (PHB)

KW - Phototrophic sulfur bacteria

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