TY - JOUR
T1 - Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence
AU - Lakshman, Dilip
AU - Roberts, Daniel P.
AU - Garrett, Wesley M.
AU - Natarajan, Savithiry S.
AU - Darwish, Omar
AU - Alkharouf, Nadim
AU - Pain, Arnab
AU - Khan, Farooq
AU - Jambhulkar, Prashant P.
AU - Mitra, Amitava
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We would like to thank S. Lakshman for a portion of the technical support.
PY - 2016/4/6
Y1 - 2016/4/6
N2 - Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. Here we used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions; the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups; and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.
AB - Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. Here we used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions; the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups; and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.
UR - http://hdl.handle.net/10754/604367
UR - http://pubs.acs.org/doi/abs/10.1021/acs.jafc.5b05735
UR - http://www.scopus.com/inward/record.url?scp=84966300032&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b05735
DO - 10.1021/acs.jafc.5b05735
M3 - Article
C2 - 27019116
SN - 0021-8561
VL - 64
SP - 3101
EP - 3110
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 15
ER -