TY - JOUR
T1 - Proteomic profiling of the brain of mice with experimental cerebral malaria
AU - Moussa, Ehab
AU - Huang, Honglei
AU - Ahras, Malika
AU - Lall, Amar
AU - Thezenas, Marie L.
AU - Fischer, Roman
AU - Kessler, Benedikt M.
AU - Pain, Arnab
AU - Billker, Oliver
AU - Casals-Pascual, Climent
N1 - KAUST Repository Item: Exported on 2023-03-16
Acknowledgements: We wish to acknowledge Stanley Barkhuysen for technical assistance. The P. berghei NK65 (New York) line was generously provided by Robert Menard, Institut Pasteur, Paris.
PY - 2018/5/8
Y1 - 2018/5/8
N2 - Cerebral malaria (CM) is a severe neurological complication of malaria infection in both adults and children. In pursuit of effective treatment of CM, clinical studies, postmortem analysis and animal models have been employed to understand the pathology and identify effective interventions. In this study, a shotgun proteomics analysis was conducted to profile the proteomic signature of the brain tissue of mice with experimental cerebral malaria (ECM) in order to further understand the underlying pathology. To identify CM-associated response, proteomic signatures of the brains of C57/Bl6N mice infected with P. berghei ANKA that developed neurological syndrome were compared to those of mice infected with P. berghei NK65 that developed equally high parasite burdens without neurological signs, and to those of non-infected mice. The results show that the CM-associated response in mice that developed neurological signs comprise mainly acute-phase reaction and coagulation cascade activation, and indicate the leakage of plasma proteins into the brain parenchyma
AB - Cerebral malaria (CM) is a severe neurological complication of malaria infection in both adults and children. In pursuit of effective treatment of CM, clinical studies, postmortem analysis and animal models have been employed to understand the pathology and identify effective interventions. In this study, a shotgun proteomics analysis was conducted to profile the proteomic signature of the brain tissue of mice with experimental cerebral malaria (ECM) in order to further understand the underlying pathology. To identify CM-associated response, proteomic signatures of the brains of C57/Bl6N mice infected with P. berghei ANKA that developed neurological syndrome were compared to those of mice infected with P. berghei NK65 that developed equally high parasite burdens without neurological signs, and to those of non-infected mice. The results show that the CM-associated response in mice that developed neurological signs comprise mainly acute-phase reaction and coagulation cascade activation, and indicate the leakage of plasma proteins into the brain parenchyma
UR - http://hdl.handle.net/10754/670065
UR - https://linkinghub.elsevier.com/retrieve/pii/S1874391917302051
UR - http://www.scopus.com/inward/record.url?scp=85020246367&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2017.06.002
DO - 10.1016/j.jprot.2017.06.002
M3 - Article
AN - SCOPUS:85020246367
SN - 1874-3919
VL - 180
SP - 61
EP - 69
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -