Protocol for characterizing strigolactones released by plant roots

Jian You Wang*, Guan Ting Erica Chen, Muhammad Jamil, Justine Braguy, Salim Sioud, Kit Xi Liew, Aparna Balakrishna, Salim Al-Babili*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

Original languageEnglish (US)
Article number101352
JournalSTAR Protocols
Issue number2
StatePublished - Jun 17 2022


  • Mass Spectrometry
  • Metabolism
  • Metabolomics
  • Model Organisms
  • Plant sciences

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)


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