TY - JOUR
T1 - Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media
AU - Astro, Veronica
AU - Ramirez Calderon, Gustavo
AU - Adamo, Antonio
N1 - KAUST Repository Item: Exported on 2023-05-10
Acknowledged KAUST grant number(s): URF/1/4012-01-01
Acknowledgements: This work was supported by a competitive research (CRG) grant number URF/1/4012-01-01 from King Abdullah University of Science and Technology to A.A.
PY - 2023/4/14
Y1 - 2023/4/14
N2 - The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1
AB - The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.1
UR - http://hdl.handle.net/10754/691592
UR - https://linkinghub.elsevier.com/retrieve/pii/S2666166723002101
UR - http://www.scopus.com/inward/record.url?scp=85152250118&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2023.102252
DO - 10.1016/j.xpro.2023.102252
M3 - Article
C2 - 37060558
SN - 2666-1667
VL - 4
SP - 102252
JO - STAR Protocols
JF - STAR Protocols
IS - 2
ER -
