This work presents a method for quantitative and multiplex detection of extracellular vesicle-derived microRNA, a promising cancer biomarker, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.1 zmol (42 aM), demonstrating its utility in clinical applications based on liquid biopsy tests.
|Title of host publication
|MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
|Chemical and Biological Microsystems Society
|Number of pages
|Published - Jan 1 2021