Rapid analysis of metanephrine and normetanephrine in urine by gas chromatography-mass spectrometry

David K. Crockett; Elizabeth L. Frank; William L. Roberts

doi:10.1093/clinchem/48.2.332

Rapid analysis of metanephrine and normetanephrine in urine by gas chromatography-mass spectrometry

David K. Crockett, Elizabeth L. Frank, William L. Roberts

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Background: Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. Methods: Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine by acid hydrolysis. To avoid the rapid decomposition of the deuterated internal standards (metanephrine-d3 and normetanephrine-d3) under hydrolysis conditions, the internal standards were added after hydrolysis. Solid-phase extraction was used to isolate the hydrolyzed metanephrines from urine. Samples were concentrated by evaporation, then derivatized simultaneously with N-methyl-N-(trimethylsilyl)trifluoroacetamide and N-methyl-bis-heptafluorobutryamide at room temperature. Results: The assay was linear from 25 to 7000 μg/L. The intraassay CVs were

Original language	English (US)
Pages (from-to)	332-337
Number of pages	6
Journal	Clinical Chemistry
Volume	48
Issue number	2
DOIs	https://doi.org/10.1093/clinchem/48.2.332
State	Published - Jan 1 2002
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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title = "Rapid analysis of metanephrine and normetanephrine in urine by gas chromatography-mass spectrometry",

abstract = "Background: Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. Methods: Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine by acid hydrolysis. To avoid the rapid decomposition of the deuterated internal standards (metanephrine-d3 and normetanephrine-d3) under hydrolysis conditions, the internal standards were added after hydrolysis. Solid-phase extraction was used to isolate the hydrolyzed metanephrines from urine. Samples were concentrated by evaporation, then derivatized simultaneously with N-methyl-N-(trimethylsilyl)trifluoroacetamide and N-methyl-bis-heptafluorobutryamide at room temperature. Results: The assay was linear from 25 to 7000 μg/L. The intraassay CVs were",

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AU - Frank, Elizabeth L.

AU - Roberts, William L.

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N2 - Background: Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. Methods: Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine by acid hydrolysis. To avoid the rapid decomposition of the deuterated internal standards (metanephrine-d3 and normetanephrine-d3) under hydrolysis conditions, the internal standards were added after hydrolysis. Solid-phase extraction was used to isolate the hydrolyzed metanephrines from urine. Samples were concentrated by evaporation, then derivatized simultaneously with N-methyl-N-(trimethylsilyl)trifluoroacetamide and N-methyl-bis-heptafluorobutryamide at room temperature. Results: The assay was linear from 25 to 7000 μg/L. The intraassay CVs were

AB - Background: Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. Methods: Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine by acid hydrolysis. To avoid the rapid decomposition of the deuterated internal standards (metanephrine-d3 and normetanephrine-d3) under hydrolysis conditions, the internal standards were added after hydrolysis. Solid-phase extraction was used to isolate the hydrolyzed metanephrines from urine. Samples were concentrated by evaporation, then derivatized simultaneously with N-methyl-N-(trimethylsilyl)trifluoroacetamide and N-methyl-bis-heptafluorobutryamide at room temperature. Results: The assay was linear from 25 to 7000 μg/L. The intraassay CVs were

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Rapid analysis of metanephrine and normetanephrine in urine by gas chromatography-mass spectrometry

Abstract

ASJC Scopus subject areas

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