Rapid analysis of metanephrine and normetanephrine in urine by gas chromatography-mass spectrometry

David K. Crockett, Elizabeth L. Frank, William L. Roberts

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Background: Widely used HPLC methods for quantification of metanephrine and normetanephrine in urine often have long analysis times and are frequently plagued by drug interferences. We describe a gas chromatography-mass spectrometry method designed to overcome these limitations. Methods: Metanephrine and normetanephrine conjugates were converted to unconjugated metanephrine and normetanephrine by acid hydrolysis. To avoid the rapid decomposition of the deuterated internal standards (metanephrine-d3 and normetanephrine-d3) under hydrolysis conditions, the internal standards were added after hydrolysis. Solid-phase extraction was used to isolate the hydrolyzed metanephrines from urine. Samples were concentrated by evaporation, then derivatized simultaneously with N-methyl-N-(trimethylsilyl)trifluoroacetamide and N-methyl-bis-heptafluorobutryamide at room temperature. Results: The assay was linear from 25 to 7000 μg/L. The intraassay CVs were
Original languageEnglish (US)
Pages (from-to)332-337
Number of pages6
JournalClinical Chemistry
Volume48
Issue number2
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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