TY - JOUR
T1 - Rapid and label-free screening of SARS-CoV-2 virus in the infected human nasopharyngeal swab samples using UiO-66-modified SPR chip
AU - Gumilar, Gilang
AU - Septiani, Ni Luh Wulan
AU - Wustoni, Shofarul
AU - Tohari, Taufik Ramdani
AU - Widayat, Wahyu
AU - Yusuf, Muhammad
AU - Wiraswati, Hesti L.
AU - Faridah, Lia
AU - Ekawardhani, Savira
AU - Anshori, Isa
AU - Yuliarto, Brian
N1 - KAUST Repository Item: Exported on 2023-08-31
Acknowledgements: This work is partially supported by the Indonesia Ministry of Education, Culture, Research, and Technology. B.Y. acknowledges financial support from Institut Teknologi Bandung, Indonesia. BY and NLWS also acknowledge financial support from Collaborative Research Center grant provided by National Research and Innovation Agency of Republic of Indonesia.
PY - 2023/7/22
Y1 - 2023/7/22
N2 - The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has become a dangerous human pathogen and caused severe illness that can lead to death. Yet, the standard practical diagnostic tools for detecting the SARS-CoV-2 virus require multi-step assay, including extraction and labeling steps. Thus, a simple and accurate detection tool is incredibly desirable to support on-site screening, preventing its spread. In this work, we developed a portable surface plasmon resonance (SPR) sensor chip modified with metal–organic frameworks (MOF) UiO-66 that serves as an immobilization matrix of single-chain variable fragment (scFv) receptors. Large surface area and good adsorption of UiO-66/scFv-modified surface lead to an improvement of binding capacity towards the protein target of the SARS-CoV-2 receptor binding domain (RBD). We further successfully demonstrated the use of this portable SPR to detect the SARS-CoV-2 virus from patients' nasopharyngeal swab samples. Interestingly, the SPR sensors can directly distinguish the positive and negative patients within 15 minutes on 23 samples, simultaneously, without the need for labeling, and the results are in line with the gold standard PCR tests. In addition, the detection limit of the sensors is 10.085 × 105 virus/mL, which follows the standard of practical screening tools. Therefore, these UiO-66 modified SPR chip sensors offer a rapid, label-free, and low-cost detection of the SARS-CoV-2 virus that can be potentially developed for other infectious diseases.
AB - The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has become a dangerous human pathogen and caused severe illness that can lead to death. Yet, the standard practical diagnostic tools for detecting the SARS-CoV-2 virus require multi-step assay, including extraction and labeling steps. Thus, a simple and accurate detection tool is incredibly desirable to support on-site screening, preventing its spread. In this work, we developed a portable surface plasmon resonance (SPR) sensor chip modified with metal–organic frameworks (MOF) UiO-66 that serves as an immobilization matrix of single-chain variable fragment (scFv) receptors. Large surface area and good adsorption of UiO-66/scFv-modified surface lead to an improvement of binding capacity towards the protein target of the SARS-CoV-2 receptor binding domain (RBD). We further successfully demonstrated the use of this portable SPR to detect the SARS-CoV-2 virus from patients' nasopharyngeal swab samples. Interestingly, the SPR sensors can directly distinguish the positive and negative patients within 15 minutes on 23 samples, simultaneously, without the need for labeling, and the results are in line with the gold standard PCR tests. In addition, the detection limit of the sensors is 10.085 × 105 virus/mL, which follows the standard of practical screening tools. Therefore, these UiO-66 modified SPR chip sensors offer a rapid, label-free, and low-cost detection of the SARS-CoV-2 virus that can be potentially developed for other infectious diseases.
UR - http://hdl.handle.net/10754/693847
UR - https://linkinghub.elsevier.com/retrieve/pii/S1226086X23004574
UR - http://www.scopus.com/inward/record.url?scp=85167820291&partnerID=8YFLogxK
U2 - 10.1016/j.jiec.2023.07.032
DO - 10.1016/j.jiec.2023.07.032
M3 - Article
SN - 1226-086X
JO - Journal of Industrial and Engineering Chemistry
JF - Journal of Industrial and Engineering Chemistry
ER -