TY - CHAP
T1 - Rapid gene isolation using MutChromSeq
AU - Steuernagel, Burkhard
AU - Vrána, Jan
AU - Karafiátová, Miroslava
AU - Wulff, Brande B.H.
AU - Doležel, Jaroslav
N1 - Generated from Scopus record by KAUST IRTS on 2023-02-20
PY - 2017/1/1
Y1 - 2017/1/1
N2 - MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
AB - MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
UR - http://link.springer.com/10.1007/978-1-4939-7249-4_20
UR - http://www.scopus.com/inward/record.url?scp=85028985949&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7249-4_20
DO - 10.1007/978-1-4939-7249-4_20
M3 - Chapter
SP - 231
EP - 243
BT - Methods in Molecular Biology
PB - Humana Press [email protected]
ER -