@inbook{88a3f608934541e8ac7427c744ef35f9,
title = "Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies",
abstract = "“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.",
keywords = "Candidate gene, Chromosome flow-sorting, Long-range scaffolding, Map-based cloning, Molecular marker",
author = "Thind, {Anupriya K.} and Thomas Wicker and Krattinger, {Simon G.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-7249-4_21",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "245--255",
booktitle = "Methods in Molecular Biology",
}