Real-time monitoring of tumour spheroid swelling under transient hypoxia using two-photon microscopy

S. M. Grist, L. Laplatine, S. S. Nasseri, A. Diaz-Gaxiola, M. C.A. Liu, L. Chrostowski, K. C. Cheung*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

We present, for the first time, real-time tracking of individual cells within 3D tumour spheroids exhibiting cyclic swelling behaviour in response to time-varying oxygen profiles. Cells and spheroids appear to reversibly swell under 0% O2. This observation was enabled by combining a microfluidic oxygen control device with two-photon microscopy (TPM) to monitor the multicellular spheroids.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages51-52
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • 3D cell culture
  • Microfluidics
  • Spheroid swelling
  • Transient/cycling hypoxia

ASJC Scopus subject areas

  • Control and Systems Engineering

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