Real-time tracking and quantification of endogenous hydrogen peroxide production in living cells using graphenated carbon nanotubes supported Prussian blue cubes

T. S.T. Balamurugan, Veerappan Mani, Chang Che Hsieh, Sheng Tung Huang, Tie Kun Peng, Hsin Yi Lin

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Recent years have seen a growing interest towards real-time tracking and quantification of reactive oxygen species such as, H2O2 released in living cells, as they are potential biomarkers for pathogenesis. Herein, we described a rapid and sensitive enzymeless H2O2 assay using Prussian blue microcubes (PB MCs) decorated graphenated carbon nanotubes (g-CNTs) modified electrode for the tracking of in-vivo H2O2 production in mammalian cells. The hydrothermally prepared PB MCs were blended with g-CNTs to yield 3D hierarchical network of PB MCs/g-CNTs nanocomposite, suitable for efficient electrocatalysis. The g-CNTs/PB MCs film modified electrode was fabricated which displayed excellent electrocatalytic activity to H2O2 reduction at minimized overpotential. A straightforward amperometric sensor was constructed that displayed working range of 25 nM–1598 μM, and detection limit of 13 nM. The described non-enzymatic H2O2 assay is rapid, sensitive, selective, durable, reproducible and robust. The method was successful in sensing in-vivo H2O2 production from Raw 264.7 cells indicating its potential in biochemical and clinical applications.
Original languageEnglish (US)
Pages (from-to)220-227
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume257
DOIs
StatePublished - Mar 1 2018
Externally publishedYes

ASJC Scopus subject areas

  • Materials Chemistry
  • Instrumentation
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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