Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Manola Moretti; Remo Proietti Zaccaria; Emiliano Descrovi; Gobind Das; Marco Leoncini; Carlo Liberale; Francesco de Angelis; Enzo Di Fabrizio

doi:10.1007/s11468-012-9385-x

Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Manola Moretti^*, Remo Proietti Zaccaria, Emiliano Descrovi, Gobind Das, Marco Leoncini, Carlo Liberale, Francesco de Angelis, Enzo Di Fabrizio

^*Corresponding author for this work

Material Science and Engineering

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 10⁵, thus revealing details down to few molecules resolution.

Original language	English (US)
Pages (from-to)	25-33
Number of pages	9
Journal	Plasmonics
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/s11468-012-9385-x
State	Published - Mar 2013

Keywords

Amyloid fibrils
Field enhancement
Raman spectroscopy
TERS
Top-illumination setup

ASJC Scopus subject areas

Biotechnology
Biophysics
Biochemistry

Access to Document

10.1007/s11468-012-9385-x

Cite this

@article{33015d7fbb8340f6a69756426e45183f,

title = "Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes",

abstract = "Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.",

keywords = "Amyloid fibrils, Field enhancement, Raman spectroscopy, TERS, Top-illumination setup",

author = "Manola Moretti and {Proietti Zaccaria}, Remo and Emiliano Descrovi and Gobind Das and Marco Leoncini and Carlo Liberale and {de Angelis}, Francesco and {Di Fabrizio}, Enzo",

note = "Funding Information: Acknowledgments The authors gratefully acknowledge support from European Projects SMD FP7 no. CP-FP 229375-2; Nanoantenna FP7 no. 241818; FOCUS FP7 no. 270483.",

year = "2013",

month = mar,

doi = "10.1007/s11468-012-9385-x",

language = "English (US)",

volume = "8",

pages = "25--33",

journal = "Plasmonics",

issn = "1557-1955",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

AU - Moretti, Manola

AU - Proietti Zaccaria, Remo

AU - Descrovi, Emiliano

AU - Das, Gobind

AU - Leoncini, Marco

AU - Liberale, Carlo

AU - de Angelis, Francesco

AU - Di Fabrizio, Enzo

N1 - Funding Information: Acknowledgments The authors gratefully acknowledge support from European Projects SMD FP7 no. CP-FP 229375-2; Nanoantenna FP7 no. 241818; FOCUS FP7 no. 270483.

PY - 2013/3

Y1 - 2013/3

N2 - Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.

AB - Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution.

KW - Amyloid fibrils

KW - Field enhancement

KW - Raman spectroscopy

KW - TERS

KW - Top-illumination setup

UR - http://www.scopus.com/inward/record.url?scp=84874432185&partnerID=8YFLogxK

U2 - 10.1007/s11468-012-9385-x

DO - 10.1007/s11468-012-9385-x

M3 - Article

AN - SCOPUS:84874432185

SN - 1557-1955

VL - 8

SP - 25

EP - 33

JO - Plasmonics

JF - Plasmonics

IS - 1

ER -

Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this