Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope

Zhoulong Zhou; Alfonso H W Ngan; Bin Tang; Anxun Wang

doi:10.1016/j.jmbbm.2011.11.010

Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope

Zhoulong Zhou, Alfonso H W Ngan, Bin Tang, Anxun Wang

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.

Original language	English (US)
Pages (from-to)	134-142
Number of pages	9
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	8
DOIs	https://doi.org/10.1016/j.jmbbm.2011.11.010
State	Published - Apr 2012

ASJC Scopus subject areas

Biomedical Engineering
Biomaterials
Mechanics of Materials

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jmbbm.2011.11.010

Cite this

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title = "Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope",

abstract = "The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. {\textcopyright} 2011 Elsevier Ltd.",

author = "Zhoulong Zhou and Ngan, {Alfonso H W} and Bin Tang and Anxun Wang",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The work done in this article was supported by a grant from the University Grants Committee of the Hong Kong Special Administration Region, PR China (Project No. SEG-HKU06).",

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AU - Zhou, Zhoulong

AU - Ngan, Alfonso H W

AU - Tang, Bin

AU - Wang, Anxun

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The work done in this article was supported by a grant from the University Grants Committee of the Hong Kong Special Administration Region, PR China (Project No. SEG-HKU06).

PY - 2012/4

Y1 - 2012/4

N2 - The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.

AB - The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed for viscoelastic materials gives elastic modulus values which are independent of the rate-jump magnitude. The results show that the rate-jump method can be used as a standard protocol for measuring elastic stiffness of living cells, since the measured values are intrinsic properties of the cells. © 2011 Elsevier Ltd.

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JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

ER -

Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope

Abstract

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