Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Shaofeng Xie; Frantisek Svec; Jean M.J. Fréchet

doi:10.1016/S0021-9673(97)00254-9

Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Shaofeng Xie, Frantisek Svec, Jean M.J. Fréchet^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns - such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery - were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.

Original language	English (US)
Pages (from-to)	65-72
Number of pages	8
Journal	Journal of Chromatography A
Volume	775
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(97)00254-9
State	Published - Jul 18 1997
Externally published	Yes

Keywords

Acrylamide
Butyl methacrylate
Methylenebisacrylamide
Monolithic columns
Proteins
Stationary phases, LC

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(97)00254-9

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title = "Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins",

abstract = "Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns - such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery - were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.",

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T1 - Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

AU - Xie, Shaofeng

AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

PY - 1997/7/18

Y1 - 1997/7/18

N2 - Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns - such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery - were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.

AB - Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns - such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery - were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.

KW - Acrylamide

KW - Butyl methacrylate

KW - Methylenebisacrylamide

KW - Monolithic columns

KW - Proteins

KW - Stationary phases, LC

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U2 - 10.1016/S0021-9673(97)00254-9

DO - 10.1016/S0021-9673(97)00254-9

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C2 - 9253195

AN - SCOPUS:0030790492

SN - 0021-9673

VL - 775

SP - 65

EP - 72

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins

Abstract

Keywords

ASJC Scopus subject areas

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