TY - JOUR
T1 - ROS-mediated vascular homeostatic control of root-to-shoot soil Na delivery in Arabidopsis
AU - Jiang, Caifu
AU - Belfield, Eric J.
AU - Mithani, Aziz
AU - Visscher, Anne
AU - Ragoussis, Jiannis
AU - Mott, Richard
AU - Smith, J. Andrew C.
AU - Harberd, Nicholas P.
N1 - KAUST Repository Item: Exported on 2021-09-21
Acknowledgements: We thank Liam Dolan for atrbohI, atrbohJ, atrbohH, atrbohE and atrbohC seeds, Jian-Kang Zhu and Yan Guo for stimulating discussion, and Xiangchao Gan for assistance with genome sequence analysis. This publication is based on the work supported by Award No. KUK-I1-002-03, made by King Abdullah University of Science and Technology, and by Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/F020759/1 and BB/F022697/1. RM and JR are supported by the Wellcome Trust grant 075491/Z/04.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2012
Y1 - 2012
N2 - Sodium (Na) is ubiquitous in soils, and is transported to plant shoots via transpiration through xylem elements in the vascular tissue. However, excess Na is damaging. Accordingly, control of xylem-sap Na concentration is important for maintenance of shoot Na homeostasis, especially under Na stress conditions. Here we report that shoot Na homeostasis of Arabidopsis thaliana plants grown in saline soils is conferred by reactive oxygen species (ROS) regulation of xylem-sap Na concentrations. We show that lack of A. thaliana respiratory burst oxidase protein F (AtrbohF; an NADPH oxidase catalysing ROS production) causes hypersensitivity of shoots to soil salinity. Lack of AtrbohF-dependent salinity-induced vascular ROS accumulation leads to increased Na concentrations in root vasculature cells and in xylem sap, thus causing delivery of damaging amounts of Na to the shoot. We also show that the excess shoot Na delivery caused by lack of AtrbohF is dependent upon transpiration. We conclude that AtrbohF increases ROS levels in wild-type root vasculature in response to raised soil salinity, thereby limiting Na concentrations in xylem sap, and in turn protecting shoot cells from transpiration-dependent delivery of excess Na. © 2012 European Molecular Biology Organization.
AB - Sodium (Na) is ubiquitous in soils, and is transported to plant shoots via transpiration through xylem elements in the vascular tissue. However, excess Na is damaging. Accordingly, control of xylem-sap Na concentration is important for maintenance of shoot Na homeostasis, especially under Na stress conditions. Here we report that shoot Na homeostasis of Arabidopsis thaliana plants grown in saline soils is conferred by reactive oxygen species (ROS) regulation of xylem-sap Na concentrations. We show that lack of A. thaliana respiratory burst oxidase protein F (AtrbohF; an NADPH oxidase catalysing ROS production) causes hypersensitivity of shoots to soil salinity. Lack of AtrbohF-dependent salinity-induced vascular ROS accumulation leads to increased Na concentrations in root vasculature cells and in xylem sap, thus causing delivery of damaging amounts of Na to the shoot. We also show that the excess shoot Na delivery caused by lack of AtrbohF is dependent upon transpiration. We conclude that AtrbohF increases ROS levels in wild-type root vasculature in response to raised soil salinity, thereby limiting Na concentrations in xylem sap, and in turn protecting shoot cells from transpiration-dependent delivery of excess Na. © 2012 European Molecular Biology Organization.
UR - http://hdl.handle.net/10754/671319
UR - http://emboj.embopress.org/cgi/doi/10.1038/emboj.2012.273
UR - http://www.scopus.com/inward/record.url?scp=84869233862&partnerID=8YFLogxK
U2 - 10.1038/emboj.2012.273
DO - 10.1038/emboj.2012.273
M3 - Article
SN - 1460-2075
VL - 31
SP - 4359
EP - 4370
JO - EMBO JOURNAL
JF - EMBO JOURNAL
IS - 22
ER -