Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels

Lara Donaldson; Ndiko Ludidi; Marc R. Knight; Chris Gehring; Katherine Denby

doi:10.1016/j.febslet.2004.06.016

Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels

Lara Donaldson, Ndiko Ludidi, Marc R. Knight, Chris Gehring, Katherine Denby^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

152 Scopus citations

Abstract

A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3^′,5^′-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (≤5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.

Original language	English (US)
Pages (from-to)	317-320
Number of pages	4
Journal	FEBS Letters
Volume	569
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.06.016
State	Published - Jul 2 2004
Externally published	Yes

Keywords

CNGCs, cyclic nucleotide gated ion channels
Cytosolic calcium
NaCl stress
Osmotic stress
cAMP, 3,5-cyclic adenosine monophosphate
cGMP
cGMP, 3,5-cyclic guanosine monophosphate

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.06.016

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title = "Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels",

abstract = "A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3′,5′-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (≤5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.",

keywords = "CNGCs, cyclic nucleotide gated ion channels, Cytosolic calcium, NaCl stress, Osmotic stress, cAMP, 3,5-cyclic adenosine monophosphate, cGMP, cGMP, 3,5-cyclic guanosine monophosphate",

author = "Lara Donaldson and Ndiko Ludidi and Knight, {Marc R.} and Chris Gehring and Katherine Denby",

note = "Funding Information: This work was partly supported by the European Commission under the Avicenne 1994 Initiative with the contract No. AVI-CT94-0010 {"}Advanced disinfection and health-care aspects of wastewater reclamation and reuse in agriculture in Mediterranean regions{"}. The authors are grateful to Cillichemie Italiana srl (Milano, Italy) for precious assistance and collaboration during the investigation.",

year = "2004",

month = jul,

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doi = "10.1016/j.febslet.2004.06.016",

language = "English (US)",

volume = "569",

pages = "317--320",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels

AU - Donaldson, Lara

AU - Ludidi, Ndiko

AU - Knight, Marc R.

AU - Gehring, Chris

AU - Denby, Katherine

N1 - Funding Information: This work was partly supported by the European Commission under the Avicenne 1994 Initiative with the contract No. AVI-CT94-0010 "Advanced disinfection and health-care aspects of wastewater reclamation and reuse in agriculture in Mediterranean regions". The authors are grateful to Cillichemie Italiana srl (Milano, Italy) for precious assistance and collaboration during the investigation.

PY - 2004/7/2

Y1 - 2004/7/2

N2 - A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3′,5′-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (≤5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.

AB - A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3′,5′-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (≤5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.

KW - CNGCs, cyclic nucleotide gated ion channels

KW - Cytosolic calcium

KW - NaCl stress

KW - Osmotic stress

KW - cAMP, 3,5-cyclic adenosine monophosphate

KW - cGMP

KW - cGMP, 3,5-cyclic guanosine monophosphate

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U2 - 10.1016/j.febslet.2004.06.016

DO - 10.1016/j.febslet.2004.06.016

M3 - Article

C2 - 15225654

AN - SCOPUS:3042646705

SN - 0014-5793

VL - 569

SP - 317

EP - 320

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels

Abstract

Keywords

ASJC Scopus subject areas

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