Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces

David Sýkora; Frantisek Svec; Jean M.J. Fréchet

doi:10.1016/S0021-9673(99)00004-7

Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces

David Sýkora, Frantisek Svec^*, Jean M.J. Fréchet

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)_12-18] and oligothymidylic acids [d(pT)_12-24] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	297-304
Number of pages	8
Journal	Journal of Chromatography A
Volume	852
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(99)00004-7
State	Published - Aug 6 1999
Externally published	Yes

Keywords

Monolithic columns
Oligonucleotides
Stationary phases, LC

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(99)00004-7

Cite this

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abstract = "Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)12-18] and oligothymidylic acids [d(pT)12-24] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. Copyright (C) 1999 Elsevier Science B.V.",

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AU - Sýkora, David

AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

PY - 1999/8/6

Y1 - 1999/8/6

N2 - Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)12-18] and oligothymidylic acids [d(pT)12-24] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. Copyright (C) 1999 Elsevier Science B.V.

AB - Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)12-18] and oligothymidylic acids [d(pT)12-24] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. Copyright (C) 1999 Elsevier Science B.V.

KW - Monolithic columns

KW - Oligonucleotides

KW - Stationary phases, LC

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U2 - 10.1016/S0021-9673(99)00004-7

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ER -

Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces

Abstract

Keywords

ASJC Scopus subject areas

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