Shear-Induced Membrane Fusion in Viscous Solutions

Maxim Kogan; Bobo Feng; Bengt Nordén; Sandra Rocha; Tamás Beke-Somfai

doi:10.1021/la404857r

Shear-Induced Membrane Fusion in Viscous Solutions

Maxim Kogan, Bobo Feng, Bengt Nordén, Sandra Rocha, Tamás Beke-Somfai

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Large unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using Förster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. © 2014 American Chemical Society.

Original language	English (US)
Pages (from-to)	4875-4878
Number of pages	4
Journal	Langmuir
Volume	30
Issue number	17
DOIs	https://doi.org/10.1021/la404857r
State	Published - Apr 23 2014
Externally published	Yes

Access to Document

10.1021/la404857r

Cite this

@article{a751e05212db47e0b9ea977103af1663,

title = "Shear-Induced Membrane Fusion in Viscous Solutions",

abstract = "Large unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using F{\"o}rster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. {\textcopyright} 2014 American Chemical Society.",

author = "Maxim Kogan and Bobo Feng and Bengt Nord{\'e}n and Sandra Rocha and Tam{\'a}s Beke-Somfai",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-11-008-23 Acknowledgements: This work was supported by King Abdullah University of Science and Technology (KAUST) (grant KUK-11-008-23), the European Research Council (EC-2008 AdG 227700-SUMO), and the Swedish Research Council - Linnaeus grant SUPRA 349-2007-8680. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2014",

month = apr,

day = "23",

doi = "10.1021/la404857r",

language = "English (US)",

volume = "30",

pages = "4875--4878",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "17",

}

TY - JOUR

T1 - Shear-Induced Membrane Fusion in Viscous Solutions

AU - Kogan, Maxim

AU - Feng, Bobo

AU - Nordén, Bengt

AU - Rocha, Sandra

AU - Beke-Somfai, Tamás

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-11-008-23 Acknowledgements: This work was supported by King Abdullah University of Science and Technology (KAUST) (grant KUK-11-008-23), the European Research Council (EC-2008 AdG 227700-SUMO), and the Swedish Research Council - Linnaeus grant SUPRA 349-2007-8680. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2014/4/23

Y1 - 2014/4/23

N2 - Large unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using Förster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. © 2014 American Chemical Society.

AB - Large unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using Förster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. © 2014 American Chemical Society.

UR - http://hdl.handle.net/10754/599358

UR - https://pubs.acs.org/doi/10.1021/la404857r

UR - http://www.scopus.com/inward/record.url?scp=84899885827&partnerID=8YFLogxK

U2 - 10.1021/la404857r

DO - 10.1021/la404857r

M3 - Article

C2 - 24758573

SN - 0743-7463

VL - 30

SP - 4875

EP - 4878

JO - Langmuir

JF - Langmuir

IS - 17

ER -

Shear-Induced Membrane Fusion in Viscous Solutions

Abstract

Access to Document

Other files and links

Fingerprint

Cite this