Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Chen Liu; Michael Malek; Ivan Poon; Lanzhou Jiang; Arif M. Siddiquee; Colin J.R. Sheppard; Ann Roberts; Harry Quiney; Douguo Zhang; Xiaocong Yuan; Jiao Lin; Christian Depeursinge; Pierre Marquet; Shan Shan Kou

doi:10.1364/PRJ.7.001042

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Chen Liu, Michael Malek, Ivan Poon, Lanzhou Jiang, Arif M. Siddiquee, Colin J.R. Sheppard, Ann Roberts, Harry Quiney, Douguo Zhang, Xiaocong Yuan, Jiao Lin, Christian Depeursinge, Pierre Marquet, Shan Shan Kou

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.

Original language	English (US)
Pages (from-to)	1042
Journal	Photonics Research
Volume	7
Issue number	9
DOIs	https://doi.org/10.1364/PRJ.7.001042
State	Published - Aug 14 2019

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10.1364/PRJ.7.001042

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Liu, C., Malek, M., Poon, I., Jiang, L., Siddiquee, A. M., Sheppard, C. J. R., Roberts, A., Quiney, H., Zhang, D., Yuan, X., Lin, J., Depeursinge, C., Marquet, P., & Kou, S. S. (2019). Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence. Photonics Research, 7(9), 1042. https://doi.org/10.1364/PRJ.7.001042

@article{6be14cd3e5844cd29fd47cb2bc2d8b5d,

title = "Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence",

abstract = "We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.",

author = "Chen Liu and Michael Malek and Ivan Poon and Lanzhou Jiang and Siddiquee, {Arif M.} and Sheppard, {Colin J.R.} and Ann Roberts and Harry Quiney and Douguo Zhang and Xiaocong Yuan and Jiao Lin and Christian Depeursinge and Pierre Marquet and Kou, {Shan Shan}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Australian Research Council (ARC) (DE120102352); National Natural Science Foundation of China (NSFC) (61427819); Shenzhen Science and Technology Innovation Commission (KQCS2015032416183980); Government of Guangdong Province (00201505); Schweizerischer Nationalfonds zur F{\"o}rderung der Wissenschaftlichen Forschung (SNF) (149652).",

year = "2019",

month = aug,

day = "14",

doi = "10.1364/PRJ.7.001042",

language = "English (US)",

volume = "7",

pages = "1042",

journal = "Photonics Research",

issn = "2327-9125",

publisher = "Optica Publishing Group (formerly OSA)",

number = "9",

}

TY - JOUR

T1 - Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

AU - Liu, Chen

AU - Malek, Michael

AU - Poon, Ivan

AU - Jiang, Lanzhou

AU - Siddiquee, Arif M.

AU - Sheppard, Colin J.R.

AU - Roberts, Ann

AU - Quiney, Harry

AU - Zhang, Douguo

AU - Yuan, Xiaocong

AU - Lin, Jiao

AU - Depeursinge, Christian

AU - Marquet, Pierre

AU - Kou, Shan Shan

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Australian Research Council (ARC) (DE120102352); National Natural Science Foundation of China (NSFC) (61427819); Shenzhen Science and Technology Innovation Commission (KQCS2015032416183980); Government of Guangdong Province (00201505); Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) (149652).

PY - 2019/8/14

Y1 - 2019/8/14

N2 - We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.

AB - We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.

UR - http://hdl.handle.net/10754/659083

UR - https://www.osapublishing.org/abstract.cfm?URI=prj-7-9-1042

UR - http://www.scopus.com/inward/record.url?scp=85073102196&partnerID=8YFLogxK

U2 - 10.1364/PRJ.7.001042

DO - 10.1364/PRJ.7.001042

M3 - Article

SN - 2327-9125

VL - 7

SP - 1042

JO - Photonics Research

JF - Photonics Research

IS - 9

ER -

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Abstract

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3D visualization of the stain-free buccal cells

3D visualization of the apoptotic Jurkat T cells

3D visualization of the stain-free neuron

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Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Abstract

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Datasets

3D visualization of the stain-free buccal cells

3D visualization of the apoptotic Jurkat T cells

3D visualization of the stain-free neuron

Cite this