TY - JOUR
T1 - Single Cell Microwave Biosensor for Monitoring Cellular Response to Electrochemotherapy
AU - Tamra, Amar
AU - Zedek, Amel
AU - Rols, Marie-Pierre
AU - Dubuc, David
AU - Grenier, Katia
N1 - KAUST Repository Item: Exported on 2022-10-27
Acknowledgements: This work was supported in part by Toulouse COMUE and Region Midi-Pyrénées, France, and in part by LAAS-CNRS micro and nano technologies platform, a member of the RENATECH French National Network.
PY - 2022/4/26
Y1 - 2022/4/26
N2 - This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells (THP-1) subjected to electrochemotherapy and obtain an electronic signature of the treatment efficiency. This biosensor proposes a non-destructive and label-free technique that first allows, with the rapid measurement of single untreated cells in their culture medium, the extraction of two frequency-dependent dielectric parameters, the capacitance (C (f)) and the conductance (G (f)). Second, this technique can powerfully reveal the effects of a chemical membrane permeabilizing treatment (Saponin). At last, it permits us to detect, and predict, the potentiation of a molecule classically used in chemotherapy (Bleomycin) when combined with the application of electric pulses (principle of electrochemotherapy). Treatment-affected cells show a decrease in the capacitive and conductive contrasts, indicating damages at the cellular levels. Along with these results, classical biological tests are conducted. Statistical analysis points out a high correlation rate (R2>0.97), which clearly reveals the reliability and efficacy of our technique and makes it an attractive technique for biology related researches and personalized medicine.
AB - This paper presents a 40 GHz microwave biosensor used to monitor and characterize single cells (THP-1) subjected to electrochemotherapy and obtain an electronic signature of the treatment efficiency. This biosensor proposes a non-destructive and label-free technique that first allows, with the rapid measurement of single untreated cells in their culture medium, the extraction of two frequency-dependent dielectric parameters, the capacitance (C (f)) and the conductance (G (f)). Second, this technique can powerfully reveal the effects of a chemical membrane permeabilizing treatment (Saponin). At last, it permits us to detect, and predict, the potentiation of a molecule classically used in chemotherapy (Bleomycin) when combined with the application of electric pulses (principle of electrochemotherapy). Treatment-affected cells show a decrease in the capacitive and conductive contrasts, indicating damages at the cellular levels. Along with these results, classical biological tests are conducted. Statistical analysis points out a high correlation rate (R2>0.97), which clearly reveals the reliability and efficacy of our technique and makes it an attractive technique for biology related researches and personalized medicine.
UR - http://hdl.handle.net/10754/685213
UR - https://ieeexplore.ieee.org/document/9763440/
UR - http://www.scopus.com/inward/record.url?scp=85129607637&partnerID=8YFLogxK
U2 - 10.1109/TBME.2022.3170267
DO - 10.1109/TBME.2022.3170267
M3 - Article
C2 - 35471894
SN - 1558-2531
SP - 1
EP - 1
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
ER -