TY - JOUR
T1 - Single-cell technologies in environmental omics
AU - Kodzius, Rimantas
AU - Gojobori, Takashi
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2015/10/25
Y1 - 2015/10/25
N2 - Environmental studies are primarily done by culturing isolated microorganisms or by amplifying and sequencing conserved genes. Difficulties understanding the complexity of large numbers of various microorganisms in an environment led to the development of techniques to enrich specific microorganisms for upstream analysis, ultimately leading to single-cell isolation and analyses. We discuss the significance of single-cell technologies in omics studies with focus on metagenomics and metatranscriptomics. We propose that by reducing sample heterogeneity using single-cell genomics, metaomic studies can be simplified.
AB - Environmental studies are primarily done by culturing isolated microorganisms or by amplifying and sequencing conserved genes. Difficulties understanding the complexity of large numbers of various microorganisms in an environment led to the development of techniques to enrich specific microorganisms for upstream analysis, ultimately leading to single-cell isolation and analyses. We discuss the significance of single-cell technologies in omics studies with focus on metagenomics and metatranscriptomics. We propose that by reducing sample heterogeneity using single-cell genomics, metaomic studies can be simplified.
UR - http://hdl.handle.net/10754/581690
UR - http://linkinghub.elsevier.com/retrieve/pii/S0378111915012391
UR - http://www.scopus.com/inward/record.url?scp=84961217857&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2015.10.031
DO - 10.1016/j.gene.2015.10.031
M3 - Article
C2 - 26474748
SN - 0378-1119
VL - 576
SP - 701
EP - 707
JO - Gene
JF - Gene
IS - 2
ER -