Single-molecule imaging with an inexpensive UV-LED light source

Akifumi Hattori; Satoshi Habuchi; Martin Vacha

doi:10.1246/cl.2009.234

Single-molecule imaging with an inexpensive UV-LED light source

Akifumi Hattori, Satoshi Habuchi, Martin Vacha^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The powerful tool of single-molecule detection and spectroscopy has so far been confined mainly to the visible spectral region. Here, we report the use of a low-cost high-power UV light emitting diode (LED) emitting at 365 nm as a light source for single-molecule detection in epi-fluorescence microscopy. The LED provides an affordable alternative to UV laser systems. The performance of the light source is demonstrated by recording fluorescence images and time-dependent fluorescence traces of single molecules of a laser dye p-bis(o-methylstyryl)benzene (bis-MSB) and of a conjugated polymer polyfluorene.

Original language	English (US)
Pages (from-to)	234-235
Number of pages	2
Journal	Chemistry Letters
Volume	38
Issue number	3
DOIs	https://doi.org/10.1246/cl.2009.234
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

General Chemistry

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AU - Vacha, Martin

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AB - The powerful tool of single-molecule detection and spectroscopy has so far been confined mainly to the visible spectral region. Here, we report the use of a low-cost high-power UV light emitting diode (LED) emitting at 365 nm as a light source for single-molecule detection in epi-fluorescence microscopy. The LED provides an affordable alternative to UV laser systems. The performance of the light source is demonstrated by recording fluorescence images and time-dependent fluorescence traces of single molecules of a laser dye p-bis(o-methylstyryl)benzene (bis-MSB) and of a conjugated polymer polyfluorene.

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Single-molecule imaging with an inexpensive UV-LED light source

Abstract

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