Smartphone-Interfaced Electrochemical Biosensor for microRNA Detection Based on Laser-Induced Graphene with π-π Stacked Peptide Nucleic Acid Probes

Sharat Chandra Barman, Muhsin Ali, Erol A. Hasan, Nimer Wehbe, Husam N. Alshareef, Dana Alsulaiman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The development of ultrasensitive, rapid, and robust point-of-care devices for biomarker detection holds great promise to revolutionize clinical diagnostics. Notably, microRNA has demonstrated significant potential as a minimally invasive cancer biomarker; however, conventional approaches for their detection require costly multistep procedures, bulky equipment, and trained personnel. Herein, we develop a peptide nucleic acid (PNA)-functionalized laser-induced graphene biosensor based on a single-step fabrication and single-step probe functionalization strategy, with a smartphone-based readout. Immobilization of a bespoke 1-pyrenebutyryl-N-end-terminated 17 mer PNA through π-π stacking on graphene was validated via Raman and X-ray photoelectron spectroscopy. As a proof-of-concept, the biosensor is applied to the detection of a prostate cancer biomarker, hsa-miR-141, exhibiting excellent analytical performance with high specificity and a limit of detection of 0.6 aM, without requiring amplification, enzymes, or thermal cycling. The versatility of this portable amplification-free platform and its ease of fabrication facilitate its translation into a point-of-care device with the potential to revolutionize clinical diagnostics, even in resource-limited settings.

Original languageEnglish (US)
Pages (from-to)837-846
Number of pages10
JournalACS Materials Letters
Volume6
Issue number3
DOIs
StatePublished - Mar 4 2024

ASJC Scopus subject areas

  • General Chemical Engineering
  • Biomedical Engineering
  • General Materials Science

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