Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay

Rafał Gierczyński; Aleksandra A. Zasada; Noura Raddadi; Maya Merabishvili; Daniele Daffonchio; Waldemar Rastawicki; Marek Jagielski

doi:10.1111/j.1574-6968.2007.00741.x

Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay

Rafał Gierczyński^*, Aleksandra A. Zasada, Noura Raddadi, Maya Merabishvili, Daniele Daffonchio, Waldemar Rastawicki, Marek Jagielski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.

Original language	English (US)
Pages (from-to)	55-59
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	272
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00741.x
State	Published - Jul 2007
Externally published	Yes

Keywords

Anthrax
Bacillus anthracis
RSI-PCR
plcR

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.2007.00741.x

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title = "Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay",

abstract = "A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.",

keywords = "Anthrax, Bacillus anthracis, RSI-PCR, plcR",

author = "Rafa{\l} Gierczy{\'n}ski and Zasada, {Aleksandra A.} and Noura Raddadi and Maya Merabishvili and Daniele Daffonchio and Waldemar Rastawicki and Marek Jagielski",

year = "2007",

month = jul,

doi = "10.1111/j.1574-6968.2007.00741.x",

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AU - Gierczyński, Rafał

AU - Zasada, Aleksandra A.

AU - Raddadi, Noura

AU - Merabishvili, Maya

AU - Daffonchio, Daniele

AU - Rastawicki, Waldemar

AU - Jagielski, Marek

PY - 2007/7

Y1 - 2007/7

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AB - A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.

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KW - Bacillus anthracis

KW - RSI-PCR

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Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay

Abstract

Keywords

ASJC Scopus subject areas

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