Structural basis for specific inhibition of the highly sensitive ShHTL7 receptor

Umar Shahul Hameed, Imran Haider, Muhammad Jamil, Boubacar Amadou Kountche, Xianrong Guo, Randa Alhassan Yahya Zarban, Dongjin Kim, Salim Al-Babili, Stefan T. Arold

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Striga hermonthica is a root parasitic plant that infests cereals, decimating yields, particularly in sub-Saharan Africa. For germination, Striga seeds require host-released strigolactones that are perceived by the family of HYPOSENSITIVE to LIGHT (ShHTL) receptors. Inhibiting seed germination would thus be a promising approach for combating Striga However, there are currently no strigolactone antagonists that specifically block ShHTLs and do not bind to DWARF14, the homologous strigolactone receptor of the host. Here, we show that the octyl phenol ethoxylate Triton X-100 inhibits S. hermonthica seed germination without affecting host plants. High-resolution X-ray structures reveal that Triton X-100 specifically plugs the catalytic pocket of ShHTL7. ShHTL7-specific inhibition by Triton X-100 demonstrates the dominant role of this particular ShHTL receptor for Striga germination. Our structural analysis provides a rationale for the broad specificity and high sensitivity of ShHTL7, and reveals that strigolactones trigger structural changes in ShHTL7 that are required for downstream signaling. Our findings identify Triton and the related 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]acetic acid as promising lead compounds for the rational design of efficient Striga-specific herbicides.
Original languageEnglish (US)
JournalEMBO reports
Issue number9
StatePublished - Jul 18 2018


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