Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint

Andreas Naschberger; Mariia Fadeeva; Daniel Klaiman; Anna Borovikova-Sheinker; Ido Caspy; Nathan Nelson; Alexey Amunts

doi:10.1038/s41477-024-01699-8

Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint

Andreas Naschberger, Mariia Fadeeva, Daniel Klaiman, Anna Borovikova-Sheinker, Ido Caspy, Nathan Nelson^*, Alexey Amunts^*

^*Corresponding author for this work

Bioscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Plant photosystem I (PSI) consists of at least 13 nuclear-encoded and 4 chloroplast-encoded subunits that together act as a sunlight-driven oxidoreductase. Here we report the structure of a PSI assembly intermediate that we isolated from greening oat seedlings. The assembly intermediate shows an absence of at least eight subunits, including PsaF and LHCI, and lacks photoreduction activity. The data show that PsaF is a regulatory checkpoint that promotes the assembly of LHCI, effectively coupling biogenesis to function.

Original language	English (US)
Pages (from-to)	874-879
Number of pages	6
Journal	Nature Plants
Volume	10
Issue number	6
DOIs	https://doi.org/10.1038/s41477-024-01699-8
State	Published - Jun 2024

ASJC Scopus subject areas

Plant Science

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10.1038/s41477-024-01699-8

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title = "Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint",

abstract = "Plant photosystem I (PSI) consists of at least 13 nuclear-encoded and 4 chloroplast-encoded subunits that together act as a sunlight-driven oxidoreductase. Here we report the structure of a PSI assembly intermediate that we isolated from greening oat seedlings. The assembly intermediate shows an absence of at least eight subunits, including PsaF and LHCI, and lacks photoreduction activity. The data show that PsaF is a regulatory checkpoint that promotes the assembly of LHCI, effectively coupling biogenesis to function.",

author = "Andreas Naschberger and Mariia Fadeeva and Daniel Klaiman and Anna Borovikova-Sheinker and Ido Caspy and Nathan Nelson and Alexey Amunts",

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AU - Naschberger, Andreas

AU - Fadeeva, Mariia

AU - Klaiman, Daniel

AU - Borovikova-Sheinker, Anna

AU - Caspy, Ido

AU - Nelson, Nathan

AU - Amunts, Alexey

PY - 2024/6

Y1 - 2024/6

N2 - Plant photosystem I (PSI) consists of at least 13 nuclear-encoded and 4 chloroplast-encoded subunits that together act as a sunlight-driven oxidoreductase. Here we report the structure of a PSI assembly intermediate that we isolated from greening oat seedlings. The assembly intermediate shows an absence of at least eight subunits, including PsaF and LHCI, and lacks photoreduction activity. The data show that PsaF is a regulatory checkpoint that promotes the assembly of LHCI, effectively coupling biogenesis to function.

AB - Plant photosystem I (PSI) consists of at least 13 nuclear-encoded and 4 chloroplast-encoded subunits that together act as a sunlight-driven oxidoreductase. Here we report the structure of a PSI assembly intermediate that we isolated from greening oat seedlings. The assembly intermediate shows an absence of at least eight subunits, including PsaF and LHCI, and lacks photoreduction activity. The data show that PsaF is a regulatory checkpoint that promotes the assembly of LHCI, effectively coupling biogenesis to function.

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U2 - 10.1038/s41477-024-01699-8

DO - 10.1038/s41477-024-01699-8

M3 - Article

C2 - 38816499

AN - SCOPUS:85194722138

SN - 2055-026X

VL - 10

SP - 874

EP - 879

JO - Nature Plants

JF - Nature Plants

IS - 6

ER -

Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint

Abstract

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